Histidine analogues of oxytocin and vasopressin as efficient ligands for Zn2+ ions - Potentiometric and NMR studies

Justyna Brasuń; Marek Cebrat; Mariusz Jaremko; Łukasz Jaremko; Olimpa Gładysz; Igor Zhukov

doi:10.1016/j.jinorgbio.2009.04.016

Histidine analogues of oxytocin and vasopressin as efficient ligands for Zn²⁺ ions - Potentiometric and NMR studies

Justyna Brasuń^*, Marek Cebrat, Mariusz Jaremko, Łukasz Jaremko, Olimpa Gładysz, Igor Zhukov

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We have characterized the interaction between the Zn²⁺ ions and the histidine analogues of oxytocin and arginine-vasopressin. Potentiometric methods were used for the determination of the stoichiometry of the complexes formed and the calculation of their stability constants. The NMR measurements revealed detailed structures of the complexes and confirmed the binding mode at physiological pH.

Original language	English (US)
Pages (from-to)	1033-1038
Number of pages	6
Journal	Journal of Inorganic Biochemistry
Volume	103
Issue number	7
DOIs	https://doi.org/10.1016/j.jinorgbio.2009.04.016
State	Published - Jul 2009
Externally published	Yes

Keywords

Disulphide bridges
Metal ion complexes
NMR structure
Oxytocin
Stability constants
Vasopressin
Zinc ions

ASJC Scopus subject areas

Biochemistry
Inorganic Chemistry

Access to Document

10.1016/j.jinorgbio.2009.04.016

Cite this

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title = "Histidine analogues of oxytocin and vasopressin as efficient ligands for Zn2+ ions - Potentiometric and NMR studies",

abstract = "We have characterized the interaction between the Zn2+ ions and the histidine analogues of oxytocin and arginine-vasopressin. Potentiometric methods were used for the determination of the stoichiometry of the complexes formed and the calculation of their stability constants. The NMR measurements revealed detailed structures of the complexes and confirmed the binding mode at physiological pH.",

keywords = "Disulphide bridges, Metal ion complexes, NMR structure, Oxytocin, Stability constants, Vasopressin, Zinc ions",

author = "Justyna Brasu{\'n} and Marek Cebrat and Mariusz Jaremko and {\L}ukasz Jaremko and Olimpa G{\l}adysz and Igor Zhukov",

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T1 - Histidine analogues of oxytocin and vasopressin as efficient ligands for Zn2+ ions - Potentiometric and NMR studies

AU - Brasuń, Justyna

AU - Cebrat, Marek

AU - Jaremko, Mariusz

AU - Jaremko, Łukasz

AU - Gładysz, Olimpa

AU - Zhukov, Igor

PY - 2009/7

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AB - We have characterized the interaction between the Zn2+ ions and the histidine analogues of oxytocin and arginine-vasopressin. Potentiometric methods were used for the determination of the stoichiometry of the complexes formed and the calculation of their stability constants. The NMR measurements revealed detailed structures of the complexes and confirmed the binding mode at physiological pH.

KW - Disulphide bridges

KW - Metal ion complexes

KW - NMR structure

KW - Oxytocin

KW - Stability constants

KW - Vasopressin

KW - Zinc ions

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DO - 10.1016/j.jinorgbio.2009.04.016

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EP - 1038

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JF - Journal of Inorganic Biochemistry

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