Identification of constitutively active AtMPK6 mutants using a functional screen in saccharomyces cerevisiae

Elodie Hudik; Souha Berriri; Heribert Hirt; Jean Colcombet

doi:10.1007/978-1-4939-0922-3_6

Identification of constitutively active AtMPK6 mutants using a functional screen in saccharomyces cerevisiae

Elodie Hudik, Souha Berriri, Heribert Hirt, Jean Colcombet

Plant Science

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1 Scopus citations

Abstract

MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed protocol for their screening, taking advantage of a functional expression assay in yeast.

Original language	English (US)
Pages (from-to)	67-77
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1171
DOIs	https://doi.org/10.1007/978-1-4939-0922-3_6
State	Published - 2014

Keywords

Constitutive activity
Functional genetic screen
In vivo cloning
MAP Kinase
Random mutagenesis
Saccharomyces cerevisiae

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-0922-3_6

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AU - Berriri, Souha

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KW - Functional genetic screen

KW - In vivo cloning

KW - MAP Kinase

KW - Random mutagenesis

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Identification of constitutively active AtMPK6 mutants using a functional screen in saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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