TY - JOUR
T1 - Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage
AU - Oosterkamp, Margreet J.
AU - Bauer, Stefan
AU - Ibáñez, Ana B.
AU - Méndez-García, Celia
AU - Hong, Pei-Ying
AU - Cann, Isaac
AU - Mackie, Roderick I.
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: We greatly appreciate the help of Sabrina Zimmerman (BP Biofuels, University of California at Berkeley, Berkeley, CA, USA), Glen Austin (BP pilot plant, Jennings, LA, USA), Michael Harland and Robert Brown (School of Chemical Sciences, Machine Shop, University of Illinois, Urbana, IL, USA), Chris Wright and Álvaro Hernández (W.M. Keck Center, Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, USA). This research was supported by the Energy Biosciences Institute (USA, project OO2J14).
PY - 2019/6/1
Y1 - 2019/6/1
N2 - The aim of this research was to identify key microorganisms for thermophilic (55 °C) anaerobic digestion of thin stillage derived from hydrolysis and ethanol fermentation of energy cane in a conventional stirred tank reactor with a 10-day hydraulic retention time. Efficient thermophilic anaerobic digestion with a specific methane production of 0.43 Lmethane/gtCOD used/d and biogas containing around 56% methane was accomplished. Due to an overnight temperature perturbation the specific methane production decreased to 0.16 Lmethane/gtCOD used/d. Analysis of the microbial community showed the importance of methanogenic Archaea belonging to Methanosarcina and Methanothermobacter as well as syntrophic Bacteria related to Thermacetogenium, Tepidanaerobacter and Anaerobaculum. This indicates that retention of biomass maintaining syntrophy and methanogenesis more efficiently may be useful for thermophilic anaerobic digestion of thin stillage derived from the production of energy cane ethanol.
AB - The aim of this research was to identify key microorganisms for thermophilic (55 °C) anaerobic digestion of thin stillage derived from hydrolysis and ethanol fermentation of energy cane in a conventional stirred tank reactor with a 10-day hydraulic retention time. Efficient thermophilic anaerobic digestion with a specific methane production of 0.43 Lmethane/gtCOD used/d and biogas containing around 56% methane was accomplished. Due to an overnight temperature perturbation the specific methane production decreased to 0.16 Lmethane/gtCOD used/d. Analysis of the microbial community showed the importance of methanogenic Archaea belonging to Methanosarcina and Methanothermobacter as well as syntrophic Bacteria related to Thermacetogenium, Tepidanaerobacter and Anaerobaculum. This indicates that retention of biomass maintaining syntrophy and methanogenesis more efficiently may be useful for thermophilic anaerobic digestion of thin stillage derived from the production of energy cane ethanol.
UR - http://hdl.handle.net/10754/660585
UR - https://linkinghub.elsevier.com/retrieve/pii/S2589014X19301446
UR - http://www.scopus.com/inward/record.url?scp=85076064752&partnerID=8YFLogxK
U2 - 10.1016/j.biteb.2019.100254
DO - 10.1016/j.biteb.2019.100254
M3 - Article
SN - 2589-014X
VL - 7
SP - 100254
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
ER -