TY - JOUR
T1 - Imaging and structural studies of DNA–protein complexes and membrane ion channels
AU - Marini, Monica
AU - Limongi, Tania
AU - Falqui, Andrea
AU - Genovese, Alessandro
AU - Allione, Marco
AU - Moretti, Manola
AU - Lopatin, Sergei
AU - Tirinato, Luca
AU - Das, Gobind
AU - Torre, Bruno
AU - Giugni, Andrea
AU - Cesca, F.
AU - Benfenati, F.
AU - Di Fabrizio, Enzo M.
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors acknowledge financial support from the KAUST start-up funding and from the Italian Ministry of Health under project no. GR-2010-2320665 and GR-2010-2311677.
PY - 2017
Y1 - 2017
N2 - In bio-imaging by electron microscopy, damage of the sample and limited contrast are the two main hurdles for reaching high image quality. We extend a new preparation method based on nanofabrication and super-hydrophobicity to the imaging and structural studies of nucleic acids, nucleic acid-protein complexes (DNA/Rad51 repair protein complex) and neuronal ion channels (gap-junction, K+ and GABA(A) channels) as paradigms of biological significance and increasing complexity. The preparation method is based on the liquid phase and is compatible with physiological conditions. Only in the very last stage, samples are dried for TEM analysis. Conventional TEM and high-resolution TEM (HRTEM) were used to achieve a resolution of 3.3 and 1.5 angstrom, respectively. The EM dataset quality allows the determination of relevant structural and metrological information on the DNA structure, DNA-protein interactions and ion channels, allowing the identification of specific macromolecules and their structure.
AB - In bio-imaging by electron microscopy, damage of the sample and limited contrast are the two main hurdles for reaching high image quality. We extend a new preparation method based on nanofabrication and super-hydrophobicity to the imaging and structural studies of nucleic acids, nucleic acid-protein complexes (DNA/Rad51 repair protein complex) and neuronal ion channels (gap-junction, K+ and GABA(A) channels) as paradigms of biological significance and increasing complexity. The preparation method is based on the liquid phase and is compatible with physiological conditions. Only in the very last stage, samples are dried for TEM analysis. Conventional TEM and high-resolution TEM (HRTEM) were used to achieve a resolution of 3.3 and 1.5 angstrom, respectively. The EM dataset quality allows the determination of relevant structural and metrological information on the DNA structure, DNA-protein interactions and ion channels, allowing the identification of specific macromolecules and their structure.
UR - http://hdl.handle.net/10754/623846
UR - http://pubs.rsc.org/en/Content/ArticleLanding/2017/NR/C6NR07958J#!divAbstract
UR - http://www.scopus.com/inward/record.url?scp=85013921202&partnerID=8YFLogxK
U2 - 10.1039/c6nr07958j
DO - 10.1039/c6nr07958j
M3 - Article
C2 - 28155926
SN - 2040-3364
VL - 9
SP - 2768
EP - 2777
JO - Nanoscale
JF - Nanoscale
IS - 8
ER -