@inbook{306a148750a3428eabddbce514b270e1,
title = "Immunocytochemistry, electron tomography, and energy dispersive X-ray spectroscopy (EDXS) on cryosections of human cancer cells doped with stimuli responsive polymeric nanogels loaded with iron oxide nanoparticles",
abstract = "The cryosectioning technique is an alternative method for preparing biological material for Transmission Electron Microscopy (TEM). We have applied this technique to study the mechanism of cell internalization of stimuli-responsive polymeric nanogels exploited as cargo nanovectors. With respect to conventional TEM processing, cryosectioning technique better preserves the morphology of solvent-sensitive nanogels and enhances the visibility of membrane-bounded organelles inside the cell cytoplasm. In this chapter we describe the protocols we have established to perform Electron Microscopy (EM)-immunocytochemistry, Electron Tomography (ET), and Energy Dispersive X-ray Spectroscopy (EDXS) chemical analysis in Scanning TEM (STEM) on cryosections of HeLa cells treated with pH-responsive nanogels hosting short interference RNA (siRNAs) and iron oxide nanoparticles (IONPs).",
keywords = "Electron tomography (ET), Energy dispersive X-ray spectroscopy (EDXS) chemical analysis, HeLa, Immunocytochemistry, Iron oxide nanoparticles (IONPs) loaded nanogel, Scanning transmission electron microscopy (STEM), Stimuli-responsive polymeric nanoparticles, Tokuyasu cryosectioning technique",
author = "Roberto Marotta and A. Falqui and A. Curcio and A. Quarta and Teresa Pellegrino",
year = "2013",
doi = "10.1007/978-1-62703-462-3_14",
language = "English (US)",
isbn = "9781627034616",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "179--198",
booktitle = "Nanomaterial Interfaces in Biology",
}
