Immunoinformatics-aided rational design of a multi-epitope vaccine targeting feline infectious peritonitis virus

Mohit Chawla, Andrés Felipe Cuspoca, Nahid Akthar, Jorge Samuel Leon Magdaleno, Siriluk Rattanabunyong, Chonticha Suwattanasophon, Nathjanan Jongkon, Kiattawee Choowongkomon*, Abdul Rajjak Shaikh*, Tabarak Malik*, Luigi Cavallo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Feline infectious peritonitis (FIP) is a grave and frequently lethal ailment instigated by feline coronavirus (FCoV) in wild and domestic feline species. The spike (S) protein of FCoV assumes a critical function in viral ingress and infection, thereby presenting a promising avenue for the development of a vaccine. In this investigation, an immunoinformatics approach was employed to ascertain immunogenic epitopes within the S-protein of FIP and formulate an innovative vaccine candidate. By subjecting the amino acid sequence of the FIP S-protein to computational scrutiny, MHC-I binding T-cell epitopes were predicted, which were subsequently evaluated for their antigenicity, toxicity, and allergenicity through in silico tools. Our analyses yielded the identification of 11 potential epitopes capable of provoking a robust immune response against FIPV. Additionally, molecular docking analysis demonstrated the ability of these epitopes to bind with feline MHC class I molecules. Through the utilization of suitable linkers, these epitopes, along with adjuvants, were integrated to design a multi-epitope vaccine candidate. Furthermore, the stability of the interaction between the vaccine candidate and feline Toll-like receptor 4 (TLR4) was established via molecular docking and molecular dynamics simulation analyses. This suggests good prospects for future experimental validation to ascertain the efficacy of our vaccine candidate in inducing a protective immune response against FIP.

Original languageEnglish (US)
Article number1280273
JournalFrontiers in Veterinary Science
Volume10
DOIs
StatePublished - 2023

Keywords

  • feline coronavirus
  • feline infectious peritonitis
  • immunoinformatics
  • reverse vaccinology
  • spike protein
  • vaccine

ASJC Scopus subject areas

  • General Veterinary

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