Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors

A. R Mohd Syaifudin; Asif I. Zia; Subhas Chandra Mukhopadhyay; Paklam Yu; Chinthaka Pasan Gooneratne; Jürgen Kosel

doi:10.1109/ICSENS.2012.6411193

Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors

A. R Mohd Syaifudin, Asif I. Zia, Subhas Chandra Mukhopadhyay, Paklam Yu, Chinthaka Pasan Gooneratne, Jürgen Kosel

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO2) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors' performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification. © 2012 IEEE.

Original language	English (US)
Title of host publication	2012 IEEE Sensors
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
ISBN (Print)	9781457717659
DOIs	https://doi.org/10.1109/ICSENS.2012.6411193
State	Published - Oct 2012

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10.1109/ICSENS.2012.6411193

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title = "Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors",

abstract = "New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO2) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors' performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification. {\textcopyright} 2012 IEEE.",

author = "Syaifudin, {A. R Mohd} and Zia, {Asif I.} and Mukhopadhyay, {Subhas Chandra} and Paklam Yu and Gooneratne, {Chinthaka Pasan} and J{\"u}rgen Kosel",

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year = "2012",

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doi = "10.1109/ICSENS.2012.6411193",

language = "English (US)",

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AU - Syaifudin, A. R Mohd

AU - Zia, Asif I.

AU - Mukhopadhyay, Subhas Chandra

AU - Yu, Paklam

AU - Gooneratne, Chinthaka Pasan

AU - Kosel, Jürgen

N1 - KAUST Repository Item: Exported on 2020-10-01

PY - 2012/10

Y1 - 2012/10

N2 - New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO2) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors' performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification. © 2012 IEEE.

AB - New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO2) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors' performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification. © 2012 IEEE.

UR - http://hdl.handle.net/10754/564615

UR - http://ieeexplore.ieee.org/document/6411193/

UR - http://www.scopus.com/inward/record.url?scp=84873975070&partnerID=8YFLogxK

U2 - 10.1109/ICSENS.2012.6411193

DO - 10.1109/ICSENS.2012.6411193

M3 - Conference contribution

SN - 9781457717659

BT - 2012 IEEE Sensors

PB - Institute of Electrical and Electronics Engineers (IEEE)

ER -

Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors

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