TY - JOUR
T1 - Inactivation and Gene Expression of a Virulent WastewaterEscherichia coliStrain and the Nonvirulent CommensalEscherichia coliDSM1103 Strain upon Solar Irradiation
AU - Aljassim, Nada I.
AU - Mantilla Calderon, David
AU - Wang, Tiannyu
AU - Hong, Pei-Ying
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): BAS/1/1033-01-01
Acknowledgements: This study is supported by KAUST baseline funding BAS/1/ 1033-01-01 awarded to P.-Y. Hong. The authors thank Dr. Shengkun Dong and Professor Helen Nguyen for providing training to N.A.-J. on the use of the solar simulator in the earlier phase of this project; Dr. Inhyuk Kwon and Professor Roderick Mackie for providing training to N.A.-J. on transcriptomics analysis; and Dr. Muhammad Raihan Jumat for providing assistance with reverse transcription. The authors express gratitude to Mr. George Princeton Dunsford for granting access to the wastewater samples and to Dr. Moataz El Ghany for ideas pertaining to E. coli transcriptomics.
PY - 2017/3/17
Y1 - 2017/3/17
N2 - This study examined the decay kinetics and molecular responses of two Escherichia coli strains upon solar irradiation. The first is E. coli PI-7, a virulent and antibiotic-resistant strain that was isolated from wastewater and carries the emerging NDM-1 antibiotic resistance gene. The other strain, E. coli DSM1103, displayed lower virulence and antibiotic resistance than E. coli PI-7. In a buffer solution, E. coli PI-7 displayed a longer lag phase prior to decay and a longer half-life compared with E. coli DSM1103 (6.64 ± 0.63 h and 2.85 ± 0.46 min vs 1.33 ± 0.52 h and 2.04 ± 0.36 min). In wastewater, both E. coli strains decayed slower than they did in buffer. Although solar irradiation remained effective in reducing the numbers of both strains by more than 5-log10 in
AB - This study examined the decay kinetics and molecular responses of two Escherichia coli strains upon solar irradiation. The first is E. coli PI-7, a virulent and antibiotic-resistant strain that was isolated from wastewater and carries the emerging NDM-1 antibiotic resistance gene. The other strain, E. coli DSM1103, displayed lower virulence and antibiotic resistance than E. coli PI-7. In a buffer solution, E. coli PI-7 displayed a longer lag phase prior to decay and a longer half-life compared with E. coli DSM1103 (6.64 ± 0.63 h and 2.85 ± 0.46 min vs 1.33 ± 0.52 h and 2.04 ± 0.36 min). In wastewater, both E. coli strains decayed slower than they did in buffer. Although solar irradiation remained effective in reducing the numbers of both strains by more than 5-log10 in
UR - http://hdl.handle.net/10754/623852
UR - http://pubs.acs.org/doi/abs/10.1021/acs.est.6b05377
UR - http://www.scopus.com/inward/record.url?scp=85019716868&partnerID=8YFLogxK
U2 - 10.1021/acs.est.6b05377
DO - 10.1021/acs.est.6b05377
M3 - Article
C2 - 28263596
SN - 0013-936X
VL - 51
SP - 3649
EP - 3659
JO - Environmental Science & Technology
JF - Environmental Science & Technology
IS - 7
ER -