TY - JOUR
T1 - Increased Antibacterial and Antibiofilm Properties of Silver Nanoparticles Using Silver Fluoride as Precursor
AU - Bertoglio, Federico
AU - De Vita, Lorenzo
AU - D’Agostino, Agnese
AU - Diaz Fernandez, Yuri
AU - Falqui, Andrea
AU - Casu, Alberto
AU - Merli, Daniele
AU - Milanese, Chiara
AU - Rossi, Silvia
AU - Taglietti, Angelo
AU - Visai, Livia
AU - Pallavicini, Piersandro
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: LDV scholarship was funded by MIUR; this research was also supported by a grant of the Italian Ministry of Education, University and Research (MIUR) to the Department of Molecular Medicine of the University of Pavia under the initiative “Dipartimenti di Eccellenza (2018–2022)”.
PY - 2020/8/3
Y1 - 2020/8/3
N2 - Silver nanoparticles were produced with AgF as the starting Ag(I) salt, with pectin as the reductant and protecting agent. While the obtained nanoparticles (pAgNP-F) have the same dimensional and physicochemical properties as those already described by us and obtained from AgNO3 and pectin (pAgNP-N), the silver nanoparticles from AgF display an increased antibacterial activity against E. coli PHL628 and Staphylococcus epidermidis RP62A (S. epidermidis RP62A), both as planktonic strains and as their biofilms with respect to pAgNP-N. In particular, a comparison of the antimicrobial and antibiofilm action of pAgNP-F has been carried out with pAgNP-N, pAgNP-N and added NaF, pure AgNO3, pure AgF, AgNO3 and added NaF and pure NaNO3 and NaF salts. By also measuring the concentration of the Ag+ cation released by pAgNP-F and pAgNP-N, we were able to unravel the separate contributions of each potential antibacterial agent, observing an evident synergy between p-AgNP and the F− anion: the F− anion increases the antibacterial power of the p-AgNP solutions even when F− is just 10 µM, a concentration at which F− alone (i.e., as its Na+ salt) is completely ineffective.
AB - Silver nanoparticles were produced with AgF as the starting Ag(I) salt, with pectin as the reductant and protecting agent. While the obtained nanoparticles (pAgNP-F) have the same dimensional and physicochemical properties as those already described by us and obtained from AgNO3 and pectin (pAgNP-N), the silver nanoparticles from AgF display an increased antibacterial activity against E. coli PHL628 and Staphylococcus epidermidis RP62A (S. epidermidis RP62A), both as planktonic strains and as their biofilms with respect to pAgNP-N. In particular, a comparison of the antimicrobial and antibiofilm action of pAgNP-F has been carried out with pAgNP-N, pAgNP-N and added NaF, pure AgNO3, pure AgF, AgNO3 and added NaF and pure NaNO3 and NaF salts. By also measuring the concentration of the Ag+ cation released by pAgNP-F and pAgNP-N, we were able to unravel the separate contributions of each potential antibacterial agent, observing an evident synergy between p-AgNP and the F− anion: the F− anion increases the antibacterial power of the p-AgNP solutions even when F− is just 10 µM, a concentration at which F− alone (i.e., as its Na+ salt) is completely ineffective.
UR - http://hdl.handle.net/10754/664538
UR - https://www.mdpi.com/1420-3049/25/15/3494
U2 - 10.3390/molecules25153494
DO - 10.3390/molecules25153494
M3 - Article
C2 - 32751978
SN - 1420-3049
VL - 25
SP - 3494
JO - Molecules
JF - Molecules
IS - 15
ER -