TY - JOUR
T1 - Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect
AU - Almohazey, Dana
AU - Ravinayagam, Vijaya
AU - Alamoudi, Widyan
AU - Akhtar, S.
AU - Dafalla, H.
AU - AlSuwaidan, Hind Nasser
AU - Almutairi, Shoruq. T.
AU - Alghamdi, Hajer Saleh
AU - Aldamen, Sukaina Ahmed
AU - Almessiere, M.A.
AU - Baykal, A.
AU - Maarouf, Ahmed A.
AU - Jermy, B. Rabindran
N1 - KAUST Repository Item: Exported on 2023-01-30
Acknowledgements: Vijaya Ravinayagam acknowledges the funding obtained from Deanship of Scientific Research, Imam Abdulrahman Bin Faisal University with grant number 2019-120-DSR. The authors would like to acknowledge Dorothy Joy H. Huelar, Janaica Yu Logan, and Edwardson G. Evangelista for their assistance in the nanocomposite preparation and in vitro studies. A. Maarouf would like to acknowledge the use of the resources of the Supercomputing Laboratory at KAUST, Saudi Arabia. B.R.J would like to acknowledge the advanced state of art facilities provided by Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University (IAU), Saudi Arabia.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2023/1/23
Y1 - 2023/1/23
N2 - In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe2O4/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe2O4/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn2+ species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt4+ species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe2O4/silica/cisplatin and MnFe2O4/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death.
AB - In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe2O4/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe2O4/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn2+ species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt4+ species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe2O4/silica/cisplatin and MnFe2O4/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death.
UR - http://hdl.handle.net/10754/687358
UR - https://www.mdpi.com/2072-6694/15/3/695
U2 - 10.3390/cancers15030695
DO - 10.3390/cancers15030695
M3 - Article
C2 - 36765654
SN - 2072-6694
VL - 15
SP - 695
JO - Cancers
JF - Cancers
IS - 3
ER -
