TY - JOUR
T1 - Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop
AU - Gedye, Kristene
AU - Gonzalez-Hernandez, Jose
AU - Ban, Yuguang
AU - Ge, Xijin
AU - Thimmapuram, Jyothi
AU - Sun, Fengjie
AU - Wright, Chris
AU - Ali, Shahjahan
AU - Boe, Arvid
AU - Owens, Vance
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors acknowledge support from the Joint USDA-DOE Feedstock Genomics program (grant# 2007-35504-18236), the Center of Excellence in Drought Tolerance through the South Dakota 2010 Initiative, and the South Dakota Agri. Exp. Station.
PY - 2010/10/1
Y1 - 2010/10/1
N2 - Prairie cordgrass (Spartina pectinata Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST-SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection.
AB - Prairie cordgrass (Spartina pectinata Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST-SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection.
UR - http://hdl.handle.net/10754/575904
UR - http://doi.wiley.com/10.3835/plantgenome2010.06.0012
U2 - 10.3835/plantgenome2010.06.0012
DO - 10.3835/plantgenome2010.06.0012
M3 - Article
SN - 1940-3372
VL - 3
SP - 69
EP - 80
JO - The Plant Genome Journal
JF - The Plant Genome Journal
IS - 2
ER -