Ionic Effects on the Equilibrium Conformation of Catenated DNA Networks

Beatrice W. Soh, Ahmed Khorshid, Dana Al Sulaiman, Patrick S. Doyle

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In this work, we study the ionic effects on the equilibrium size and shape of kinetoplasts, a two-dimensional (2D) network of catenated DNA rings. With increasing ionic strength from 5 to 200 mM, we observe a decrease in kinetoplast size, primarily driven by the long-range electrostatic interactions that give rise to a change in effective DNA width. A fit of the experimentally measured kinetoplast size versus effective width yields a scaling exponent of 0.38. To probe the quantitative effects of ionic strength on kinetoplast size, we develop a scaling argument based on a generalized Flory approach for a 2D polymer represented as monomers on an open lattice. Interestingly, while ionic strength has a significant effect on kinetoplast size, we find that it does not impact the kinetoplast shape.
Original languageEnglish (US)
Pages (from-to)8502-8508
Number of pages7
JournalMacromolecules
Volume53
Issue number19
DOIs
StatePublished - Oct 13 2020
Externally publishedYes

ASJC Scopus subject areas

  • Materials Chemistry
  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry

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