Isolation and characterization of a phosphoprotein phosphatase type 2A gene from alfalfa

Manfred Pirck; Aniko Páy; Erwin Heberle-Bors; Heribert Hirt

doi:10.1007/BF00276891

Isolation and characterization of a phosphoprotein phosphatase type 2A gene from alfalfa

Manfred Pirck, Aniko Páy, Erwin Heberle-Bors, Heribert Hirt^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Phosphoprotein phosphatases are central regulatory components of the cell cycle in eukaryotes. We report the cloning and sequencing of an alfalfa phosphoprotein phosphatase type 2A (pp2aMs) cDNA. The predicted protein sequence shows high similarity to PP2A from Brassica napus, rabbit and Drosophila. No changes in pp2aMs mRNA abundance during the cell cycle were found. During growth of a batch cell culture, mRNA levels decreased gradually. In planta, all organs contained pp2a transcripts but maximal mRNA levels were detected in stems. Since Southern analysis indicated the presence of a small pp2a gene family in alfalfa, it appears that different subtypes may have specialized roles in various tissues and developmental situations which await characterization.

Original language	English (US)
Pages (from-to)	126-131
Number of pages	6
Journal	MGG Molecular & General Genetics
Volume	240
Issue number	1
DOIs	https://doi.org/10.1007/BF00276891
State	Published - Jul 1993
Externally published	Yes

Keywords

Cell cycle
Medicago sativa
Mitosis
Phosphatase
Phosphorylation

ASJC Scopus subject areas

Genetics

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10.1007/BF00276891

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title = "Isolation and characterization of a phosphoprotein phosphatase type 2A gene from alfalfa",

abstract = "Phosphoprotein phosphatases are central regulatory components of the cell cycle in eukaryotes. We report the cloning and sequencing of an alfalfa phosphoprotein phosphatase type 2A (pp2aMs) cDNA. The predicted protein sequence shows high similarity to PP2A from Brassica napus, rabbit and Drosophila. No changes in pp2aMs mRNA abundance during the cell cycle were found. During growth of a batch cell culture, mRNA levels decreased gradually. In planta, all organs contained pp2a transcripts but maximal mRNA levels were detected in stems. Since Southern analysis indicated the presence of a small pp2a gene family in alfalfa, it appears that different subtypes may have specialized roles in various tissues and developmental situations which await characterization.",

keywords = "Cell cycle, Medicago sativa, Mitosis, Phosphatase, Phosphorylation",

author = "Manfred Pirck and Aniko P{\'a}y and Erwin Heberle-Bors and Heribert Hirt",

year = "1993",

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doi = "10.1007/BF00276891",

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T1 - Isolation and characterization of a phosphoprotein phosphatase type 2A gene from alfalfa

AU - Pirck, Manfred

AU - Páy, Aniko

AU - Heberle-Bors, Erwin

AU - Hirt, Heribert

PY - 1993/7

Y1 - 1993/7

N2 - Phosphoprotein phosphatases are central regulatory components of the cell cycle in eukaryotes. We report the cloning and sequencing of an alfalfa phosphoprotein phosphatase type 2A (pp2aMs) cDNA. The predicted protein sequence shows high similarity to PP2A from Brassica napus, rabbit and Drosophila. No changes in pp2aMs mRNA abundance during the cell cycle were found. During growth of a batch cell culture, mRNA levels decreased gradually. In planta, all organs contained pp2a transcripts but maximal mRNA levels were detected in stems. Since Southern analysis indicated the presence of a small pp2a gene family in alfalfa, it appears that different subtypes may have specialized roles in various tissues and developmental situations which await characterization.

AB - Phosphoprotein phosphatases are central regulatory components of the cell cycle in eukaryotes. We report the cloning and sequencing of an alfalfa phosphoprotein phosphatase type 2A (pp2aMs) cDNA. The predicted protein sequence shows high similarity to PP2A from Brassica napus, rabbit and Drosophila. No changes in pp2aMs mRNA abundance during the cell cycle were found. During growth of a batch cell culture, mRNA levels decreased gradually. In planta, all organs contained pp2a transcripts but maximal mRNA levels were detected in stems. Since Southern analysis indicated the presence of a small pp2a gene family in alfalfa, it appears that different subtypes may have specialized roles in various tissues and developmental situations which await characterization.

KW - Cell cycle

KW - Medicago sativa

KW - Mitosis

KW - Phosphatase

KW - Phosphorylation

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U2 - 10.1007/BF00276891

DO - 10.1007/BF00276891

M3 - Article

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AN - SCOPUS:0027248126

SN - 0026-8925

VL - 240

SP - 126

EP - 131

JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

IS - 1

ER -

Isolation and characterization of a phosphoprotein phosphatase type 2A gene from alfalfa

Abstract

Keywords

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