Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

F. Alberto; L. Correia; C. Billot; C. M. Duarte; E. Serrão

doi:10.1046/j.1471-8286.2003.00463.x

Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

F. Alberto^*, L. Correia, C. Billot, C. M. Duarte, E. Serrão

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.

Original language	English (US)
Pages (from-to)	397-399
Number of pages	3
Journal	Molecular Ecology Notes
Volume	3
Issue number	3
DOIs	https://doi.org/10.1046/j.1471-8286.2003.00463.x
State	Published - Sep 2003
Externally published	Yes

Keywords

Clonal plant
Cymodocea nodosa
Microsatellites
Seagrass

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1046/j.1471-8286.2003.00463.x

Cite this

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title = "Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa",

abstract = "In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.",

keywords = "Clonal plant, Cymodocea nodosa, Microsatellites, Seagrass",

author = "F. Alberto and L. Correia and C. Billot and Duarte, {C. M.} and E. Serr{\~a}o",

year = "2003",

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doi = "10.1046/j.1471-8286.2003.00463.x",

language = "English (US)",

volume = "3",

pages = "397--399",

journal = "Molecular Ecology Notes",

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T1 - Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

AU - Alberto, F.

AU - Correia, L.

AU - Billot, C.

AU - Duarte, C. M.

AU - Serrão, E.

PY - 2003/9

Y1 - 2003/9

N2 - In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.

AB - In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.

KW - Clonal plant

KW - Cymodocea nodosa

KW - Microsatellites

KW - Seagrass

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DO - 10.1046/j.1471-8286.2003.00463.x

M3 - Article

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SN - 1471-8278

VL - 3

SP - 397

EP - 399

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 3

ER -

Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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