Isolation of Elaeagnus-compatible Frankia from soils collected in Tunisia

Maher Gtari; Lorenzo Brusetti; Gharbi Skander; Diego Mora; Abdellatif Boudabous; Daniele Daffonchio

doi:10.1016/j.femsle.2004.04.001

Isolation of Elaeagnus-compatible Frankia from soils collected in Tunisia

Maher Gtari, Lorenzo Brusetti, Gharbi Skander, Diego Mora, Abdellatif Boudabous, Daniele Daffonchio^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The occurrence and diversity of Frankia nodulating Elaeagnus angustifolia in Tunisia were evaluated in 30 soils from different regions by a Frankia-capturing assay. Despite the absence of actinorhizal plants in 24 of the 30 soils, nodules were captured from all the samples. Eight pure strains were isolated from single colonies grown in agar medium. On the basis of 16S rRNA and GlnII sequences, seven strains were clustered with Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic groups while one strain described a new lineage in the Frankia assemblage, indicating that Frankia strains genetically diverse from previously known Elaeagnus-infective strains are present in tunisian soils. Genomic fingerprinting determined by rep-PCR, and tDNA-PCR-SSCP, confirmed the wide genetic diversity of the strains.

Original language	English (US)
Pages (from-to)	349-355
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	234
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2004.04.001
State	Published - May 15 2004
Externally published	Yes

Keywords

Elaeagnus
Frankia
Genetic diversity
Isolated strains
Soil
Tunisia

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/j.femsle.2004.04.001

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@article{8758ece5fae84c02892c80044afbca12,

title = "Isolation of Elaeagnus-compatible Frankia from soils collected in Tunisia",

abstract = "The occurrence and diversity of Frankia nodulating Elaeagnus angustifolia in Tunisia were evaluated in 30 soils from different regions by a Frankia-capturing assay. Despite the absence of actinorhizal plants in 24 of the 30 soils, nodules were captured from all the samples. Eight pure strains were isolated from single colonies grown in agar medium. On the basis of 16S rRNA and GlnII sequences, seven strains were clustered with Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic groups while one strain described a new lineage in the Frankia assemblage, indicating that Frankia strains genetically diverse from previously known Elaeagnus-infective strains are present in tunisian soils. Genomic fingerprinting determined by rep-PCR, and tDNA-PCR-SSCP, confirmed the wide genetic diversity of the strains.",

keywords = "Elaeagnus, Frankia, Genetic diversity, Isolated strains, Soil, Tunisia",

author = "Maher Gtari and Lorenzo Brusetti and Gharbi Skander and Diego Mora and Abdellatif Boudabous and Daniele Daffonchio",

note = "Funding Information: MG was supported by a grant from the Ministere de l'Enseignement superieur et de la Recherche Scientifique et Technologique of Tunisia.",

year = "2004",

month = may,

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doi = "10.1016/j.femsle.2004.04.001",

language = "English (US)",

volume = "234",

pages = "349--355",

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AU - Gtari, Maher

AU - Brusetti, Lorenzo

AU - Skander, Gharbi

AU - Mora, Diego

AU - Boudabous, Abdellatif

AU - Daffonchio, Daniele

N1 - Funding Information: MG was supported by a grant from the Ministere de l'Enseignement superieur et de la Recherche Scientifique et Technologique of Tunisia.

PY - 2004/5/15

Y1 - 2004/5/15

N2 - The occurrence and diversity of Frankia nodulating Elaeagnus angustifolia in Tunisia were evaluated in 30 soils from different regions by a Frankia-capturing assay. Despite the absence of actinorhizal plants in 24 of the 30 soils, nodules were captured from all the samples. Eight pure strains were isolated from single colonies grown in agar medium. On the basis of 16S rRNA and GlnII sequences, seven strains were clustered with Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic groups while one strain described a new lineage in the Frankia assemblage, indicating that Frankia strains genetically diverse from previously known Elaeagnus-infective strains are present in tunisian soils. Genomic fingerprinting determined by rep-PCR, and tDNA-PCR-SSCP, confirmed the wide genetic diversity of the strains.

AB - The occurrence and diversity of Frankia nodulating Elaeagnus angustifolia in Tunisia were evaluated in 30 soils from different regions by a Frankia-capturing assay. Despite the absence of actinorhizal plants in 24 of the 30 soils, nodules were captured from all the samples. Eight pure strains were isolated from single colonies grown in agar medium. On the basis of 16S rRNA and GlnII sequences, seven strains were clustered with Frankia, colonizing Elaeagnaceae and Rhamnaceae in two different phylogenetic groups while one strain described a new lineage in the Frankia assemblage, indicating that Frankia strains genetically diverse from previously known Elaeagnus-infective strains are present in tunisian soils. Genomic fingerprinting determined by rep-PCR, and tDNA-PCR-SSCP, confirmed the wide genetic diversity of the strains.

KW - Elaeagnus

KW - Frankia

KW - Genetic diversity

KW - Isolated strains

KW - Soil

KW - Tunisia

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U2 - 10.1016/j.femsle.2004.04.001

DO - 10.1016/j.femsle.2004.04.001

M3 - Article

C2 - 15135543

AN - SCOPUS:2342572774

SN - 0378-1097

VL - 234

SP - 349

EP - 355

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Isolation of Elaeagnus-compatible Frankia from soils collected in Tunisia

Abstract

Keywords

ASJC Scopus subject areas

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