Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

Sung Sick Woo; Vipin K. Rastogi; Hong Bin Zhang; Andrew H. Paterson; Keith F. Schertz; Rod A. Wing

doi:10.1007/BF02668396

Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

Sung Sick Woo, Vipin K. Rastogi, Hong Bin Zhang, Andrew H. Paterson, Keith F. Schertz, Rod A. Wing

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries. © 1995 Kluwer Academic Publishers.

Original language	English (US)
Journal	Plant Molecular Biology Reporter
Volume	13
Issue number	1
DOIs	https://doi.org/10.1007/BF02668396
State	Published - Mar 1 1995
Externally published	Yes

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10.1007/BF02668396

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title = "Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction",

abstract = "A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries. {\textcopyright} 1995 Kluwer Academic Publishers.",

author = "Woo, {Sung Sick} and Rastogi, {Vipin K.} and Zhang, {Hong Bin} and Paterson, {Andrew H.} and Schertz, {Keith F.} and Wing, {Rod A.}",

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doi = "10.1007/BF02668396",

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T1 - Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

AU - Woo, Sung Sick

AU - Rastogi, Vipin K.

AU - Zhang, Hong Bin

AU - Paterson, Andrew H.

AU - Schertz, Keith F.

AU - Wing, Rod A.

N1 - Generated from Scopus record by KAUST IRTS on 2019-11-20

PY - 1995/3/1

Y1 - 1995/3/1

N2 - A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries. © 1995 Kluwer Academic Publishers.

AB - A method was developed for the isolation of megabase-size DNA from Sorghum bicolor. Sorghum protoplasts were isolated from young leaf tissue, embedded in an agarose matrix as microbeads or plugs, followed by cell lysis and protein degradation. The DNA prepared by this method was larger than 1 Mb in size and readily digestible with restriction enzymes. The DNA was shown to be suitable for physical mapping, and was successfully used for the construction of BAC and YAC libraries. © 1995 Kluwer Academic Publishers.

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VL - 13

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 1

ER -

Isolation of megabase-size DNA from sorghum and applications for physical mapping and bacterial and yeast artificial chromosome library construction

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