Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane

L. J. Heraty; M. E. Fuller; L. Huang; Teofilo Abrajano; N. C. Sturchio

doi:10.1016/S0146-6380(99)00062-5

Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane

L. J. Heraty^*, M. E. Fuller, L. Huang, Teofilo Abrajano, N. C. Sturchio

^*Corresponding author for this work

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Abstract

Microbial culture experiments were performed at 22°C to investigate isotopic fractionation of carbon (C) and chlorine (Cl) during aerobic degradation of dichloromethane (DCM) by MC8b, a gram-negative methylotrophic organism closely related to the genera Methylobacterium or Ochrobactrum. Values of the isotopic fractionation factor, α, were estimated from the experimental results. These values are 0.9576 ± 0 0015 for C and 0.9962 ± 0.0003 for Cl, corresponding to kinetic isotope effects (KIE) of 1,0424 for C and 1 0038 for Cl. The C isotope effect accompanying aerobic degradation of DCM by MC8b is roughly twice that resulting from microbial oxidation of CH₄. Further studies of this type, for a variety of chlorinated aliphatic hydrocarbons (CAHs) under a range of biogeochemical conditions, may find important applications in the characterization and remediation of sites contaminated with CAHs.

Original language	English (US)
Pages (from-to)	793-799
Number of pages	7
Journal	Organic Geochemistry
Volume	30
Issue number	8 A
DOIs	https://doi.org/10.1016/S0146-6380(99)00062-5
State	Published - Aug 1 1999

ASJC Scopus subject areas

Geochemistry and Petrology

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10.1016/S0146-6380(99)00062-5

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title = "Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane",

abstract = "Microbial culture experiments were performed at 22°C to investigate isotopic fractionation of carbon (C) and chlorine (Cl) during aerobic degradation of dichloromethane (DCM) by MC8b, a gram-negative methylotrophic organism closely related to the genera Methylobacterium or Ochrobactrum. Values of the isotopic fractionation factor, α, were estimated from the experimental results. These values are 0.9576 ± 0 0015 for C and 0.9962 ± 0.0003 for Cl, corresponding to kinetic isotope effects (KIE) of 1,0424 for C and 1 0038 for Cl. The C isotope effect accompanying aerobic degradation of DCM by MC8b is roughly twice that resulting from microbial oxidation of CH4. Further studies of this type, for a variety of chlorinated aliphatic hydrocarbons (CAHs) under a range of biogeochemical conditions, may find important applications in the characterization and remediation of sites contaminated with CAHs.",

author = "Heraty, {L. J.} and Fuller, {M. E.} and L. Huang and Teofilo Abrajano and Sturchio, {N. C.}",

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T1 - Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane

AU - Heraty, L. J.

AU - Fuller, M. E.

AU - Huang, L.

AU - Abrajano, Teofilo

AU - Sturchio, N. C.

PY - 1999/8/1

Y1 - 1999/8/1

N2 - Microbial culture experiments were performed at 22°C to investigate isotopic fractionation of carbon (C) and chlorine (Cl) during aerobic degradation of dichloromethane (DCM) by MC8b, a gram-negative methylotrophic organism closely related to the genera Methylobacterium or Ochrobactrum. Values of the isotopic fractionation factor, α, were estimated from the experimental results. These values are 0.9576 ± 0 0015 for C and 0.9962 ± 0.0003 for Cl, corresponding to kinetic isotope effects (KIE) of 1,0424 for C and 1 0038 for Cl. The C isotope effect accompanying aerobic degradation of DCM by MC8b is roughly twice that resulting from microbial oxidation of CH4. Further studies of this type, for a variety of chlorinated aliphatic hydrocarbons (CAHs) under a range of biogeochemical conditions, may find important applications in the characterization and remediation of sites contaminated with CAHs.

AB - Microbial culture experiments were performed at 22°C to investigate isotopic fractionation of carbon (C) and chlorine (Cl) during aerobic degradation of dichloromethane (DCM) by MC8b, a gram-negative methylotrophic organism closely related to the genera Methylobacterium or Ochrobactrum. Values of the isotopic fractionation factor, α, were estimated from the experimental results. These values are 0.9576 ± 0 0015 for C and 0.9962 ± 0.0003 for Cl, corresponding to kinetic isotope effects (KIE) of 1,0424 for C and 1 0038 for Cl. The C isotope effect accompanying aerobic degradation of DCM by MC8b is roughly twice that resulting from microbial oxidation of CH4. Further studies of this type, for a variety of chlorinated aliphatic hydrocarbons (CAHs) under a range of biogeochemical conditions, may find important applications in the characterization and remediation of sites contaminated with CAHs.

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EP - 799

JO - Organic Geochemistry

JF - Organic Geochemistry

IS - 8 A

ER -

Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane

Abstract

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