Jasmonates induce intracellular alkalinization and closure of Paphiopedilum guard cells

C. A. Gehring; H. R. Irving; R. McConchie; R. W. Parish

doi:10.1006/anbo.1997.0471

Jasmonates induce intracellular alkalinization and closure of Paphiopedilum guard cells

C. A. Gehring, H. R. Irving, R. McConchie, R. W. Parish^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

Jasmonates (jasmonic acid or methyl jasmonate) promote stomatal closure in Paphiopedilum Supersuk (RHS, 1973) and P, tonsum (Rchb.f) Stein. Studies on guard cells loaded with pH dependent fluorescent dyes show that jasmonates cause intracellular alkalinization of up to 0.5 pH units within 5 to 15 min. Jasmonate-induced alkalinization always preceded stomatal closure and where alkalinization was not detected no closure occurred. Propionic acid inhibited jasmonate-induced stomatal closure, suggesting that jasmonate-induced intracellular alkalinization is involved in guard cell movements.

Original language	English (US)
Pages (from-to)	485-489
Number of pages	5
Journal	Annals of botany
Volume	80
Issue number	4
DOIs	https://doi.org/10.1006/anbo.1997.0471
State	Published - Oct 1997
Externally published	Yes

Keywords

BCECF
Confocal microscopy
Cytosolic pH
Guard cells
Jasmonic acid
Methyl jasmonate
Paphiopedilum Supersuk (R. H. S.)
Snarf-1
Stomatal movements

ASJC Scopus subject areas

General Medicine

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10.1006/anbo.1997.0471

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title = "Jasmonates induce intracellular alkalinization and closure of Paphiopedilum guard cells",

abstract = "Jasmonates (jasmonic acid or methyl jasmonate) promote stomatal closure in Paphiopedilum Supersuk (RHS, 1973) and P, tonsum (Rchb.f) Stein. Studies on guard cells loaded with pH dependent fluorescent dyes show that jasmonates cause intracellular alkalinization of up to 0.5 pH units within 5 to 15 min. Jasmonate-induced alkalinization always preceded stomatal closure and where alkalinization was not detected no closure occurred. Propionic acid inhibited jasmonate-induced stomatal closure, suggesting that jasmonate-induced intracellular alkalinization is involved in guard cell movements.",

keywords = "BCECF, Confocal microscopy, Cytosolic pH, Guard cells, Jasmonic acid, Methyl jasmonate, Paphiopedilum Supersuk (R. H. S.), Snarf-1, Stomatal movements",

author = "Gehring, {C. A.} and Irving, {H. R.} and R. McConchie and Parish, {R. W.}",

year = "1997",

month = oct,

doi = "10.1006/anbo.1997.0471",

language = "English (US)",

volume = "80",

pages = "485--489",

journal = "Annals of botany",

issn = "0305-7364",

publisher = "Oxford University Press",

number = "4",

}

TY - JOUR

T1 - Jasmonates induce intracellular alkalinization and closure of Paphiopedilum guard cells

AU - Gehring, C. A.

AU - Irving, H. R.

AU - McConchie, R.

AU - Parish, R. W.

PY - 1997/10

Y1 - 1997/10

N2 - Jasmonates (jasmonic acid or methyl jasmonate) promote stomatal closure in Paphiopedilum Supersuk (RHS, 1973) and P, tonsum (Rchb.f) Stein. Studies on guard cells loaded with pH dependent fluorescent dyes show that jasmonates cause intracellular alkalinization of up to 0.5 pH units within 5 to 15 min. Jasmonate-induced alkalinization always preceded stomatal closure and where alkalinization was not detected no closure occurred. Propionic acid inhibited jasmonate-induced stomatal closure, suggesting that jasmonate-induced intracellular alkalinization is involved in guard cell movements.

AB - Jasmonates (jasmonic acid or methyl jasmonate) promote stomatal closure in Paphiopedilum Supersuk (RHS, 1973) and P, tonsum (Rchb.f) Stein. Studies on guard cells loaded with pH dependent fluorescent dyes show that jasmonates cause intracellular alkalinization of up to 0.5 pH units within 5 to 15 min. Jasmonate-induced alkalinization always preceded stomatal closure and where alkalinization was not detected no closure occurred. Propionic acid inhibited jasmonate-induced stomatal closure, suggesting that jasmonate-induced intracellular alkalinization is involved in guard cell movements.

KW - BCECF

KW - Confocal microscopy

KW - Cytosolic pH

KW - Guard cells

KW - Jasmonic acid

KW - Methyl jasmonate

KW - Paphiopedilum Supersuk (R. H. S.)

KW - Snarf-1

KW - Stomatal movements

UR - http://www.scopus.com/inward/record.url?scp=0030819937&partnerID=8YFLogxK

U2 - 10.1006/anbo.1997.0471

DO - 10.1006/anbo.1997.0471

M3 - Article

AN - SCOPUS:0030819937

SN - 0305-7364

VL - 80

SP - 485

EP - 489

JO - Annals of botany

JF - Annals of botany

IS - 4

ER -

Jasmonates induce intracellular alkalinization and closure of Paphiopedilum guard cells

Abstract

Keywords

ASJC Scopus subject areas

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