TY - JOUR
T1 - Kristallstruktur der Methylornithin-Synthase (PylB): Einblicke in die Biosynthese von Pyrrolysin
AU - Quitterer, Felix
AU - List, Anja
AU - Eisenreich, Wolfgang
AU - Bacher, Adelbert
AU - Groll, Michael
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): FIC/2010/07
Acknowledgements: Wir danken Sophie Vieweg fur ihre experimentelle Unterstutzung und den Mitarbeitern der PXI des Paul Scherrer Instituts, Swiss Light Source (Villigen, Schweiz) fur ihre Hilfe beim Messen der Datensutze. Diese Arbeit wurde von der Hans-Fischer-Gesellschaft und der King Abdullah University of Science and Technology (Award No. FIC/2010/07) gefçrdert.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2011/11/16
Y1 - 2011/11/16
N2 - Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.
AB - Made by the barrel load: The biosynthetic pathway of the recently discovered 22nd amino acid, pyrrolysine, starts with an isomerization of lysine to methylornithine, catalyzed by PylB. The X-ray crystal structure of PylB is determined (see picture) and shows it has a TIM barrel fold. The sealed central cavity contains a [4Fe-4S] cluster, S-adenosylmethionine (SAM), and methylornithine, whose 2R,3R configuration could be confirmed. The data suggest a fragmentation–recombination mechanism via a glycyl radical intermediate.
UR - http://hdl.handle.net/10754/598689
UR - http://doi.wiley.com/10.1002/ange.201106765
U2 - 10.1002/ange.201106765
DO - 10.1002/ange.201106765
M3 - Article
SN - 0044-8249
VL - 124
SP - 1367
EP - 1370
JO - Angewandte Chemie
JF - Angewandte Chemie
IS - 6
ER -