Lab on a chip automates in vitro cell culturing

Gerardo Perozziello; Jacob Møllenbach; Steen Laursen; Enzo Di Fabrizio; Krist Gernaey; Ulrich Krühne

doi:10.1016/j.mee.2012.07.027

Lab on a chip automates in vitro cell culturing

Gerardo Perozziello^*, Jacob Møllenbach, Steen Laursen, Enzo Di Fabrizio, Krist Gernaey, Ulrich Krühne

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20 μl/h. A common pH electrode is supplied by 14 μl sample, which is expanded with help of DI water.

Original language	English (US)
Pages (from-to)	655-658
Number of pages	4
Journal	Microelectronic Engineering
Volume	98
DOIs	https://doi.org/10.1016/j.mee.2012.07.027
State	Published - Oct 2012
Externally published	Yes

Keywords

Automated in vitro fertilization
Lab on a chip
Rapid prototyping
Single cell cultivation
T and pH control

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics
Surfaces, Coatings and Films
Electrical and Electronic Engineering

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10.1016/j.mee.2012.07.027

Cite this

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title = "Lab on a chip automates in vitro cell culturing",

abstract = "A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20 μl/h. A common pH electrode is supplied by 14 μl sample, which is expanded with help of DI water.",

keywords = "Automated in vitro fertilization, Lab on a chip, Rapid prototyping, Single cell cultivation, T and pH control",

author = "Gerardo Perozziello and Jacob M{\o}llenbach and Steen Laursen and {Di Fabrizio}, Enzo and Krist Gernaey and Ulrich Kr{\"u}hne",

year = "2012",

month = oct,

doi = "10.1016/j.mee.2012.07.027",

language = "English (US)",

volume = "98",

pages = "655--658",

journal = "Microelectronic Engineering",

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T1 - Lab on a chip automates in vitro cell culturing

AU - Perozziello, Gerardo

AU - Møllenbach, Jacob

AU - Laursen, Steen

AU - Di Fabrizio, Enzo

AU - Gernaey, Krist

AU - Krühne, Ulrich

PY - 2012/10

Y1 - 2012/10

N2 - A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20 μl/h. A common pH electrode is supplied by 14 μl sample, which is expanded with help of DI water.

AB - A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20 μl/h. A common pH electrode is supplied by 14 μl sample, which is expanded with help of DI water.

KW - Automated in vitro fertilization

KW - Lab on a chip

KW - Rapid prototyping

KW - Single cell cultivation

KW - T and pH control

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DO - 10.1016/j.mee.2012.07.027

M3 - Article

AN - SCOPUS:84865593492

SN - 0167-9317

VL - 98

SP - 655

EP - 658

JO - Microelectronic Engineering

JF - Microelectronic Engineering

ER -

Lab on a chip automates in vitro cell culturing

Abstract

Keywords

ASJC Scopus subject areas

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