Label-free detection of alanine aminotransferase using a graphene field-effect biosensor

Jim Long Her; Tung Ming Pan; Wan Ying Lin; Kai Sheng Wang; Lain Jong Li

doi:10.1016/j.snb.2013.03.026

Label-free detection of alanine aminotransferase using a graphene field-effect biosensor

Jim Long Her, Tung Ming Pan^*, Wan Ying Lin, Kai Sheng Wang, Lain Jong Li

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

In this paper, we developed a low operation voltage and a single reaction step of a graphene field-effect transistor (GFET) biosensor device to detect alanine aminotransferase (ALT). The device is fabricated using large-area graphene thin films by means of chemical vapor deposition. The GFET device operated at 0.1 V drain voltage and -0.1 to 0.3 V sweep gate voltage exhibited a high pH sensitivity of 23.12 mV/pH, a low hysteresis voltage of 1.2 mV, and a small drift rate of 4.74 mV/h. In this research, the hybrid configuration of l-alanine and α-ketoglutarate immobilized on the graphene membrane was proved to be able to detect ALT with good linearity (R² = 0.99) in the ALT concentration range of 10-100 U/L.

Original language	English (US)
Pages (from-to)	396-400
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	182
DOIs	https://doi.org/10.1016/j.snb.2013.03.026
State	Published - 2013
Externally published	Yes

Keywords

Alanine aminotransferase (ALT)
Graphene field-effect transistor (GFET)
l-Alanine
α-Ketoglutarate

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Metals and Alloys
Instrumentation
Materials Chemistry
Surfaces, Coatings and Films
Electrical and Electronic Engineering

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10.1016/j.snb.2013.03.026

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@article{935070e177434e699e1f3956df333e81,

title = "Label-free detection of alanine aminotransferase using a graphene field-effect biosensor",

abstract = "In this paper, we developed a low operation voltage and a single reaction step of a graphene field-effect transistor (GFET) biosensor device to detect alanine aminotransferase (ALT). The device is fabricated using large-area graphene thin films by means of chemical vapor deposition. The GFET device operated at 0.1 V drain voltage and -0.1 to 0.3 V sweep gate voltage exhibited a high pH sensitivity of 23.12 mV/pH, a low hysteresis voltage of 1.2 mV, and a small drift rate of 4.74 mV/h. In this research, the hybrid configuration of l-alanine and α-ketoglutarate immobilized on the graphene membrane was proved to be able to detect ALT with good linearity (R2 = 0.99) in the ALT concentration range of 10-100 U/L.",

keywords = "Alanine aminotransferase (ALT), Graphene field-effect transistor (GFET), l-Alanine, α-Ketoglutarate",

author = "Her, {Jim Long} and Pan, {Tung Ming} and Lin, {Wan Ying} and Wang, {Kai Sheng} and Li, {Lain Jong}",

note = "Funding Information: This work was supported by the National Science Council, Taiwan, Republic of China , under contract no. NSC-97-2221-E-182-050-MY3 . ",

year = "2013",

doi = "10.1016/j.snb.2013.03.026",

language = "English (US)",

volume = "182",

pages = "396--400",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

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TY - JOUR

T1 - Label-free detection of alanine aminotransferase using a graphene field-effect biosensor

AU - Her, Jim Long

AU - Pan, Tung Ming

AU - Lin, Wan Ying

AU - Wang, Kai Sheng

AU - Li, Lain Jong

N1 - Funding Information: This work was supported by the National Science Council, Taiwan, Republic of China , under contract no. NSC-97-2221-E-182-050-MY3 .

PY - 2013

Y1 - 2013

N2 - In this paper, we developed a low operation voltage and a single reaction step of a graphene field-effect transistor (GFET) biosensor device to detect alanine aminotransferase (ALT). The device is fabricated using large-area graphene thin films by means of chemical vapor deposition. The GFET device operated at 0.1 V drain voltage and -0.1 to 0.3 V sweep gate voltage exhibited a high pH sensitivity of 23.12 mV/pH, a low hysteresis voltage of 1.2 mV, and a small drift rate of 4.74 mV/h. In this research, the hybrid configuration of l-alanine and α-ketoglutarate immobilized on the graphene membrane was proved to be able to detect ALT with good linearity (R2 = 0.99) in the ALT concentration range of 10-100 U/L.

AB - In this paper, we developed a low operation voltage and a single reaction step of a graphene field-effect transistor (GFET) biosensor device to detect alanine aminotransferase (ALT). The device is fabricated using large-area graphene thin films by means of chemical vapor deposition. The GFET device operated at 0.1 V drain voltage and -0.1 to 0.3 V sweep gate voltage exhibited a high pH sensitivity of 23.12 mV/pH, a low hysteresis voltage of 1.2 mV, and a small drift rate of 4.74 mV/h. In this research, the hybrid configuration of l-alanine and α-ketoglutarate immobilized on the graphene membrane was proved to be able to detect ALT with good linearity (R2 = 0.99) in the ALT concentration range of 10-100 U/L.

KW - Alanine aminotransferase (ALT)

KW - Graphene field-effect transistor (GFET)

KW - l-Alanine

KW - α-Ketoglutarate

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U2 - 10.1016/j.snb.2013.03.026

DO - 10.1016/j.snb.2013.03.026

M3 - Article

AN - SCOPUS:84876146969

SN - 0925-4005

VL - 182

SP - 396

EP - 400

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Label-free detection of alanine aminotransferase using a graphene field-effect biosensor

Abstract

Keywords

ASJC Scopus subject areas

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