Laccase-membrane reactors for decolorization of an acid azo dye in aqueous phase: Process optimization

Krishna P. Katuri*, S. Venkata Mohan, S. Sridhar, B. R. Pati, P. N. Sarma

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

89 Scopus citations


In the present investigation, performance of various laccase-membrane reactor configurations including direct enzyme contact, enzyme impregnated, immobilized enzyme and a reactor system based on laccase immobilization in chitosan membranes for decolorization of azo dye (acid black 10 BX) were examined using laccase enzyme purified from white rot fungi Pleurotus ostreatus 1804. A five-step laccase purification procedure was employed, which improved the enzymatic activity by 8.27 folds. Laccase was confirmed by comparing with the standard marker using SDS-PAGE electrophoresis, which showed molecular weight of 63 kDa. Experimental data showed that laccase has great potential for color removal without addition of external redox mediators. Various process parameters viz. aqueous phase of pH 6.0, enzyme concentration of 1.75 U/ml, dye concentration of 20 mg/L, temperature of 30 °C and reaction time of 120 min were optimized to achieve maximum decolorization efficiencies. Moreover, different laccase-membrane reactor configurations were tested to determine the efficacy of repeated application of laccase on dye decolorization process. Among the different reactor configurations employed, laccase encapsulated in chitosan membrane showed advantages such as short-term contact period and reusability of enzyme for a number of cycles.

Original languageEnglish (US)
Pages (from-to)3647-3658
Number of pages12
JournalWater research
Issue number15
StatePublished - Aug 2009
Externally publishedYes


  • Azo dye
  • Decolorization
  • Enzyme membrane reactors
  • Enzyme purification
  • Laccase
  • Parameters optimization

ASJC Scopus subject areas

  • Water Science and Technology
  • Ecological Modeling
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering
  • Civil and Structural Engineering


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