TY - JOUR
T1 - LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses
AU - Mahas, Ahmed
AU - Hassan, Norhan
AU - Aman, Rashid
AU - Maršić, Tin
AU - Wang, Qiaochu
AU - Ali, Zahir
AU - Mahfouz, Magdy M.
N1 - KAUST Repository Item: Exported on 2021-03-30
Acknowledged KAUST grant number(s): CRG8-URF/4026
Acknowledgements: This research was supported by the King Abdullah University of Science and Technology (KAUST) Competitive Research Grants (CRG8) under Award No. CRG8-URF/4026.
PY - 2021/3/12
Y1 - 2021/3/12
N2 - One important factor for successful disease management is the ability to rapidly and accurately identify the causal agent. Plant viruses cause severe economic losses and pose a serious threat to sustainable agriculture. Therefore, optimization of the speed, sensitivity, feasibility, portability, and accuracy of virus detection is urgently needed. Here, we developed a clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid diagnostic method utilizing the CRISPR–Cas12a system for detecting two geminiviruses, tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV), which have single-stranded DNA genomes. Our assay detected TYLCV and ToLCNDV in infected plants with high sensitivity and specificity. Our newly developed assay can be performed in ~1 h and provides easy-to-interpret visual readouts using a simple, low-cost fluorescence visualizer, making it suitable for point-of-use applications.
AB - One important factor for successful disease management is the ability to rapidly and accurately identify the causal agent. Plant viruses cause severe economic losses and pose a serious threat to sustainable agriculture. Therefore, optimization of the speed, sensitivity, feasibility, portability, and accuracy of virus detection is urgently needed. Here, we developed a clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid diagnostic method utilizing the CRISPR–Cas12a system for detecting two geminiviruses, tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV), which have single-stranded DNA genomes. Our assay detected TYLCV and ToLCNDV in infected plants with high sensitivity and specificity. Our newly developed assay can be performed in ~1 h and provides easy-to-interpret visual readouts using a simple, low-cost fluorescence visualizer, making it suitable for point-of-use applications.
UR - http://hdl.handle.net/10754/668355
UR - https://www.mdpi.com/1999-4915/13/3/466
U2 - 10.3390/v13030466
DO - 10.3390/v13030466
M3 - Article
C2 - 33808947
SN - 1999-4915
VL - 13
SP - 466
JO - Viruses
JF - Viruses
IS - 3
ER -
