TY - JOUR
T1 - Laser-scribed graphene sensor based on gold nanostructures and molecularly imprinted polymers: Application for Her-2 cancer biomarker detection
AU - Lahcen, Abdellatif Ait
AU - Rauf, Sakandar
AU - Aljedaibi, Abdulrahman
AU - De Oliveira Filho, José Ilton
AU - Beduk, Tutku
AU - Mani, Veerappan
AU - Alsharee, Husam N.
AU - Salama, Khaled N.
N1 - KAUST Repository Item: Exported on 2021-08-11
Acknowledged KAUST grant number(s): KAUST Sensor Initiative
Acknowledgements: The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative and KAUST visiting student program for supporting this work. We would like to thank Saravanan Yuvaraja for his support for AFM characterization.
PY - 2021/8
Y1 - 2021/8
N2 - Laser scribed graphene (LSG) has shown great potential as a sensing platform due to its high sensitivity, simplicity, porosity, and flexibility. In this context, we report a novel biosensing platform that utilizes LSG electrodes modified with nanostructured gold and molecularly imprinted polymer (MIP) to enhance its sensitivity and selectivity. This biomimetic sensing platform is used to detect the human epidermal growth factor receptor 2 (Her-2) protein, a significant breast cancer biomarker. Hence, a simple and accurate biomimetic sensor is developed in this study. To the best of our knowledge, this is the first report on nanostructured gold modified MIP-based LSG sensor for Her-2. LSG electrodes are fabricated by irradiation of a polyimide sheet using a CO2 laser. Nanostructured gold is electrodeposited onto the LSG to enhance its sensitivity and facilitate better Her-2 immobilization on the sensor surface. For MIP preparation, 3, 4-ethylenedioxythiophene (EDOT) was electropolymerized after pre-adsorption of Her-2 on the electrode surface for 20 min. The MIP deposition, removal, and adsorption parameters were investigated and optimized. The developed sensing strategy showed an excellent ability to detect Her-2 in the concentration range from 1 to 200 ng/mL with a LOD of 0.43 ng/mL. The biomimetic sensor showed high selectivity towards the detection of Her-2 in the presence of other interfering molecules and appreciable recovery values of Her-2 in the spiked undiluted human serum samples. Finally, to show the potential application of the developed LSG-AuNS-MIP sensor as a point-of-care device, the sensor is integrated with a homemade open-source electrochemical analyzer KAUSTat to detect Her-2.
AB - Laser scribed graphene (LSG) has shown great potential as a sensing platform due to its high sensitivity, simplicity, porosity, and flexibility. In this context, we report a novel biosensing platform that utilizes LSG electrodes modified with nanostructured gold and molecularly imprinted polymer (MIP) to enhance its sensitivity and selectivity. This biomimetic sensing platform is used to detect the human epidermal growth factor receptor 2 (Her-2) protein, a significant breast cancer biomarker. Hence, a simple and accurate biomimetic sensor is developed in this study. To the best of our knowledge, this is the first report on nanostructured gold modified MIP-based LSG sensor for Her-2. LSG electrodes are fabricated by irradiation of a polyimide sheet using a CO2 laser. Nanostructured gold is electrodeposited onto the LSG to enhance its sensitivity and facilitate better Her-2 immobilization on the sensor surface. For MIP preparation, 3, 4-ethylenedioxythiophene (EDOT) was electropolymerized after pre-adsorption of Her-2 on the electrode surface for 20 min. The MIP deposition, removal, and adsorption parameters were investigated and optimized. The developed sensing strategy showed an excellent ability to detect Her-2 in the concentration range from 1 to 200 ng/mL with a LOD of 0.43 ng/mL. The biomimetic sensor showed high selectivity towards the detection of Her-2 in the presence of other interfering molecules and appreciable recovery values of Her-2 in the spiked undiluted human serum samples. Finally, to show the potential application of the developed LSG-AuNS-MIP sensor as a point-of-care device, the sensor is integrated with a homemade open-source electrochemical analyzer KAUSTat to detect Her-2.
UR - http://hdl.handle.net/10754/670543
UR - https://linkinghub.elsevier.com/retrieve/pii/S0925400521011242
U2 - 10.1016/j.snb.2021.130556
DO - 10.1016/j.snb.2021.130556
M3 - Article
SN - 0925-4005
SP - 130556
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -
