Latex-functionalized monolithic columns for the separation of carbohydrates by micro anion-exchange chromatography

Emily F. Hilder, Frantisek Svec, Jean M.J. Fréchet

Research output: Contribution to journalArticlepeer-review

112 Scopus citations


A novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2- acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalJournal of Chromatography A
Issue number1-2 SPEC. ISS.
StatePublished - Oct 22 2004


  • Carbohydrates
  • Latex
  • Monolithic columns
  • Stationary phases, LC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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