TY - JOUR
T1 - Light harvesting complexes of Chromera velia, photosynthetic relative of apicomplexan parasites
AU - Tichý, Josef
AU - Gardian, Zdenko
AU - Bína, David
AU - Koník, Peter
AU - Litvín, Radek V.
AU - Herbstová, Miroslava
AU - Pain, Arnab
AU - Vácha, František
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): IC/2010/09
Acknowledgements: This research was supported by the GAAV 32-IAA601410907 and GAJU 134/2010/P with institutional support RVO:60077344. Research on C. velia genomics is supported by an Award IC/2010/09 made by the King Abdullah University of Science and Technology (KAUST) to A. Pain. The authors are greatly indebted to the team of J. Lukes, R. Sobotka and P. Keeling, for giving access to their data concerning the split of the photosystem I reaction center protein prior to publication. Thanks are extended to I. Hunalova and F. Matousek for technical assistance.
PY - 2013/6
Y1 - 2013/6
N2 - The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for "Chromera Light Harvesting" complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. © 2013 Elsevier B.V. All rights reserved.
AB - The structure and composition of the light harvesting complexes from the unicellular alga Chromera velia were studied by means of optical spectroscopy, biochemical and electron microscopy methods. Two different types of antennae systems were identified. One exhibited a molecular weight (18-19 kDa) similar to FCP (fucoxanthin chlorophyll protein) complexes from diatoms, however, single particle analysis and circular dichroism spectroscopy indicated similarity of this structure to the recently characterized XLH antenna of xanthophytes. In light of these data we denote this antenna complex CLH, for "Chromera Light Harvesting" complex. The other system was identified as the photosystem I with bound Light Harvesting Complexes (PSI-LHCr) related to the red algae LHCI antennae. The result of this study is the finding that C. velia, when grown in natural light conditions, possesses light harvesting antennae typically found in two different, evolutionary distant, groups of photosynthetic organisms. © 2013 Elsevier B.V. All rights reserved.
UR - http://hdl.handle.net/10754/562781
UR - https://linkinghub.elsevier.com/retrieve/pii/S0005272813000285
UR - http://www.scopus.com/inward/record.url?scp=84875933753&partnerID=8YFLogxK
U2 - 10.1016/j.bbabio.2013.02.002
DO - 10.1016/j.bbabio.2013.02.002
M3 - Article
C2 - 23428396
SN - 0005-2728
VL - 1827
SP - 723
EP - 729
JO - Biochimica et Biophysica Acta (BBA) - Bioenergetics
JF - Biochimica et Biophysica Acta (BBA) - Bioenergetics
IS - 6
ER -