TY - JOUR
T1 - Lipid droplets and small extracellular vesicles
T2 - More than two independent entities
AU - Genard, Géraldine C.
AU - Tirinato, Luca
AU - Pagliari, Francesca
AU - Da Silva, Jessica
AU - Giammona, Alessandro
AU - Alquraish, Fatema
AU - Reyes, Maria Parra
AU - Bordas, Marie
AU - Marafioti, Maria Grazia
AU - Franco, Simone Di
AU - Janssen, Jeannette
AU - Garcia-Calderón, Daniel
AU - Hanley, Rachel
AU - Nistico, Clelia
AU - Fukasawa, Yoshinori
AU - Müller, Torsten
AU - Krijgsveld, Jeroen
AU - Todaro, Matilde
AU - Costanzo, Francesco Saverio
AU - Stassi, Giorgio
AU - Nessling, Michelle
AU - Richter, Karsten
AU - Maass, Kendra K.
AU - Liberale, Carlo
AU - Seco, Joao
N1 - Publisher Copyright:
© 2024 The Author(s). Journal of Extracellular Biology published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.
PY - 2024/9
Y1 - 2024/9
N2 - Despite increasing knowledge about small extracellular vesicle (sEV) composition and functions in cell–cell communication, the mechanism behind their biogenesis remains unclear. Here, we reveal for the first time that sEV biogenesis and release into the microenvironment are tightly connected with another important organelle, Lipid Droplets (LDs). The correlation was observed in several human cancer cell lines as well as patient-derived colorectal cancer stem cells (CR-CSCs). Our results demonstrated that external stimuli such as radiation, pH, hypoxia or lipid-interfering drugs, known to affect the number of LDs/cell, similarly influenced sEV secretion. Importantly, through multiple omics data, at both mRNA and protein levels, we revealed RAB5C as a potential important molecular player behind this organelle connection. Altogether, the potential to fine-tune sEV biogenesis by targeting LDs could significantly impact the amount, cargos and properties of these sEVs, opening new clinical perspectives.
AB - Despite increasing knowledge about small extracellular vesicle (sEV) composition and functions in cell–cell communication, the mechanism behind their biogenesis remains unclear. Here, we reveal for the first time that sEV biogenesis and release into the microenvironment are tightly connected with another important organelle, Lipid Droplets (LDs). The correlation was observed in several human cancer cell lines as well as patient-derived colorectal cancer stem cells (CR-CSCs). Our results demonstrated that external stimuli such as radiation, pH, hypoxia or lipid-interfering drugs, known to affect the number of LDs/cell, similarly influenced sEV secretion. Importantly, through multiple omics data, at both mRNA and protein levels, we revealed RAB5C as a potential important molecular player behind this organelle connection. Altogether, the potential to fine-tune sEV biogenesis by targeting LDs could significantly impact the amount, cargos and properties of these sEVs, opening new clinical perspectives.
KW - exosomes
KW - hypoxia
KW - iron metabolism
KW - irradiation
KW - lipid droplets
KW - pH
KW - Rab
KW - small extracellular vesicles
UR - http://www.scopus.com/inward/record.url?scp=85208446441&partnerID=8YFLogxK
U2 - 10.1002/jex2.162
DO - 10.1002/jex2.162
M3 - Article
C2 - 39257626
AN - SCOPUS:85208446441
SN - 2768-2811
VL - 3
JO - Journal of Extracellular Biology
JF - Journal of Extracellular Biology
IS - 9
M1 - e162
ER -
