Abstract
BACKGROUND: Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography - tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement of androstenedione, dehydroepiandrosterone (DHEA), and testosterone in these patients. METHODS: We spiked 200 μL of serum or plasma with isotope-labeled internal standards and performed extraction with methyl t-butyl ether. We then derivatized the extracts with hydroxylamine and analyzed them by LC-MS/MS using a 2-dimensional chromatographic separation with a 3.5-min analysis time. RESULTS: Total imprecision for each analyte was
Original language | English (US) |
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Pages (from-to) | 1138-1147 |
Number of pages | 10 |
Journal | Clinical Chemistry |
Volume | 56 |
Issue number | 7 |
DOIs | |
State | Published - Jul 1 2010 |
Externally published | Yes |
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical