Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

Mark M. Kushnir; Alan L. Rockwood; William L. Roberts; Bingfang Yue; Jonas Bergquist; A. Wayne Meikle

doi:10.1016/j.clinbiochem.2010.07.008

Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

Mark M. Kushnir, Alan L. Rockwood, William L. Roberts, Bingfang Yue, Jonas Bergquist, A. Wayne Meikle

Research output: Contribution to journal › Article › peer-review

200 Scopus citations

Abstract

Liquid chromatography tandem mass spectrometry is one of the most specific techniques available in clinical laboratories. In the past, immunoassays were the primary methodology for analysis of steroids in biological samples because they are rapid and easy to perform. However, these methods were shown to suffer from the lack of specificity for measuring many of the diagnostically important steroids. LC-MS/MS overcomes many of the limitations of immunoassays, enhances diagnostic utility of the testing, and expands diagnostic capabilities in endocrinology. In addition to the superior quality of the measurements, LC-MS/MS allows high throughput testing using small sample volume with minimal sample preparation, and frees the laboratory from dependence on suppliers of assay specific reagents. LC-MS/MS is being widely employed for routine measurement of steroids, and the methodology plays an important role in the standardization and harmonization of measurements among clinical laboratories. © 2010 The Canadian Society of Clinical Chemists.

Original language	English (US)
Pages (from-to)	77-88
Number of pages	12
Journal	Clinical Biochemistry
Volume	44
Issue number	1
DOIs	https://doi.org/10.1016/j.clinbiochem.2010.07.008
State	Published - Jan 1 2011
Externally published	Yes

ASJC Scopus subject areas

Clinical Biochemistry

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10.1016/j.clinbiochem.2010.07.008

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title = "Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories",

abstract = "Liquid chromatography tandem mass spectrometry is one of the most specific techniques available in clinical laboratories. In the past, immunoassays were the primary methodology for analysis of steroids in biological samples because they are rapid and easy to perform. However, these methods were shown to suffer from the lack of specificity for measuring many of the diagnostically important steroids. LC-MS/MS overcomes many of the limitations of immunoassays, enhances diagnostic utility of the testing, and expands diagnostic capabilities in endocrinology. In addition to the superior quality of the measurements, LC-MS/MS allows high throughput testing using small sample volume with minimal sample preparation, and frees the laboratory from dependence on suppliers of assay specific reagents. LC-MS/MS is being widely employed for routine measurement of steroids, and the methodology plays an important role in the standardization and harmonization of measurements among clinical laboratories. {\textcopyright} 2010 The Canadian Society of Clinical Chemists.",

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AU - Rockwood, Alan L.

AU - Roberts, William L.

AU - Yue, Bingfang

AU - Bergquist, Jonas

AU - Meikle, A. Wayne

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AB - Liquid chromatography tandem mass spectrometry is one of the most specific techniques available in clinical laboratories. In the past, immunoassays were the primary methodology for analysis of steroids in biological samples because they are rapid and easy to perform. However, these methods were shown to suffer from the lack of specificity for measuring many of the diagnostically important steroids. LC-MS/MS overcomes many of the limitations of immunoassays, enhances diagnostic utility of the testing, and expands diagnostic capabilities in endocrinology. In addition to the superior quality of the measurements, LC-MS/MS allows high throughput testing using small sample volume with minimal sample preparation, and frees the laboratory from dependence on suppliers of assay specific reagents. LC-MS/MS is being widely employed for routine measurement of steroids, and the methodology plays an important role in the standardization and harmonization of measurements among clinical laboratories. © 2010 The Canadian Society of Clinical Chemists.

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Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

Abstract

ASJC Scopus subject areas

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