Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

Silvia Nappini; Tamer Al Kayal; Debora Berti; Bengt Nordèn; Piero Baglioni

doi:10.1021/jz2000936

Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

Silvia Nappini, Tamer Al Kayal, Debora Berti, Bengt Nordèn, Piero Baglioni

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Abstract

Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. © 2011 American Chemical Society.

Original language	English (US)
Pages (from-to)	713-718
Number of pages	6
Journal	The Journal of Physical Chemistry Letters
Volume	2
Issue number	7
DOIs	https://doi.org/10.1021/jz2000936
State	Published - Mar 10 2011
Externally published	Yes

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@article{ef26599867af4cadb334384abb2e8f0d,

title = "Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy",

abstract = "Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. {\textcopyright} 2011 American Chemical Society.",

author = "Silvia Nappini and {Al Kayal}, Tamer and Debora Berti and Bengt Nord{\`e}n and Piero Baglioni",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Financial support from CSGI, MIUR-PRIN (2008-20087K9A2J), and FIRB (RBPR05JH2P ITALNANONET) is acknowledged. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr. Tore Eriksson is acknowledged for building the alternating magnetic field generator. This publication acknowledges KAUST support, but has no KAUST affiliated authors.",

year = "2011",

month = mar,

day = "10",

doi = "10.1021/jz2000936",

language = "English (US)",

volume = "2",

pages = "713--718",

journal = "The Journal of Physical Chemistry Letters",

issn = "1948-7185",

publisher = "American Chemical Society",

number = "7",

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T1 - Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

AU - Nappini, Silvia

AU - Al Kayal, Tamer

AU - Berti, Debora

AU - Nordèn, Bengt

AU - Baglioni, Piero

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: Financial support from CSGI, MIUR-PRIN (2008-20087K9A2J), and FIRB (RBPR05JH2P ITALNANONET) is acknowledged. B.N. acknowledges a King Abdullah University of Science and Technology Award making this project possible. Dr. Tore Eriksson is acknowledged for building the alternating magnetic field generator. This publication acknowledges KAUST support, but has no KAUST affiliated authors.

PY - 2011/3/10

Y1 - 2011/3/10

N2 - Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. © 2011 American Chemical Society.

AB - Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMF). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO 2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO 2(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse. © 2011 American Chemical Society.

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U2 - 10.1021/jz2000936

DO - 10.1021/jz2000936

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JO - The Journal of Physical Chemistry Letters

JF - The Journal of Physical Chemistry Letters

IS - 7

ER -

Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy

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