Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation

Valerio Orlando*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

411 Scopus citations


Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events. Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalTrends in Biochemical Sciences
Issue number3
StatePublished - Mar 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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