Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation

Valerio Orlando

doi:10.1016/S0968-0004(99)01535-2

Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation

Valerio Orlando^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

411 Scopus citations

Abstract

Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events. Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material. Copyright (C) 2000 Elsevier Science Ltd.

Original language	English (US)
Pages (from-to)	99-104
Number of pages	6
Journal	Trends in Biochemical Sciences
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/S0968-0004(99)01535-2
State	Published - Mar 1 2000
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/S0968-0004(99)01535-2

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title = "Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation",

abstract = "Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events. Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material. Copyright (C) 2000 Elsevier Science Ltd.",

author = "Valerio Orlando",

note = "Funding Information: I would like to thank Andreas Hecht and Michael Grunstein for providing their original data, Bhavin Parekh and Tom Maniatis for sharing technical information prior to publication, Renato Paro and members of his laboratory for having shared the navigation in the X-CHIP hyperspace, and Paul Orban for proofreading the manuscript. This work is supported by the Associazione Italiana Ricerca sul Cancro and Telethon.",

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PY - 2000/3/1

Y1 - 2000/3/1

N2 - Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events. Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material. Copyright (C) 2000 Elsevier Science Ltd.

AB - Gene regulation is a complex process. Numerous factors appear to be required for the accurate temporal and spatial regulation of each gene. Often these factors are assembled into multiprotein complexes, contributing to specific gene regulation events. Understanding how all these factors are organized in the chromosome and how their function is regulated in vivo is a challenging task. One of the most useful techniques for studying this level of gene regulation is the in vivo fixation by formaldehyde crosslinking of proteins to proteins and proteins to DNA, followed by immunoprecipitation of the fixed material. Copyright (C) 2000 Elsevier Science Ltd.

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DO - 10.1016/S0968-0004(99)01535-2

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AN - SCOPUS:0034161329

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Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation

Abstract

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