TY - JOUR
T1 - Marine-derived collagen biomaterials from echinoderm connective tissues
AU - Ferrario, Cinzia
AU - Leggio, Livio
AU - Leone, Roberta
AU - Di Benedetto, Cristiano
AU - Guidetti, Luca
AU - Coccè, Valentina
AU - Ascagni, Miriam
AU - Bonasoro, Francesco
AU - La Porta, Caterina A.M.
AU - Candia Carnevali, M. Daniela
AU - Sugni, Michela
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This research was funded by Young Researcher Grant (University of Milan, PI: Dr. Michela Sugni). We are grateful to the Marine Protected Area of Portofino (GE, Italy) for permission to collect experimental animals and to the scuba diver Dr. Dario Fassini for helping during the collection. We would like to deeply thank Dr. Paolo Tremolada for his support with statistical analyses, Andrea Spalletti and Silvia Colombo for helping in cell culture experiments, Prof. Iain Wilkie for mechanical equipment use and Dr. Dario Fassini for welcome comments on mechanical tests. We also thank Dr. Elena Canciani for providing commercial membranes used in this work.
PY - 2016/4/2
Y1 - 2016/4/2
N2 - The use of marine collagens is a hot topic in the field of tissue engineering. Echinoderms possess unique connective tissues (Mutable Collagenous Tissues, MCTs) which can represent an innovative source of collagen to develop collagen barrier-membranes for Guided Tissue Regeneration (GTR). In the present work we used MCTs from different echinoderm models (sea urchin, starfish and sea cucumber) to produce echinoderm-derived collagen membranes (EDCMs). Commercial membranes for GTR or soluble/reassembled (fibrillar) bovine collagen substrates were used as controls. The three EDCMs were similar among each other in terms of structure and mechanical performances and were much thinner and mechanically more resistant than the commercial membranes. Number of fibroblasts seeded on sea-urchin membranes were comparable to the bovine collagen substrates. Cell morphology on all EDCMs was similar to that of structurally comparable (reassembled) bovine collagen substrates. Overall, echinoderms, and sea urchins particularly, are alternative collagen sources to produce efficient GTR membranes. Sea urchins display a further advantage in terms of eco-sustainability by recycling tissues from food wastes.
AB - The use of marine collagens is a hot topic in the field of tissue engineering. Echinoderms possess unique connective tissues (Mutable Collagenous Tissues, MCTs) which can represent an innovative source of collagen to develop collagen barrier-membranes for Guided Tissue Regeneration (GTR). In the present work we used MCTs from different echinoderm models (sea urchin, starfish and sea cucumber) to produce echinoderm-derived collagen membranes (EDCMs). Commercial membranes for GTR or soluble/reassembled (fibrillar) bovine collagen substrates were used as controls. The three EDCMs were similar among each other in terms of structure and mechanical performances and were much thinner and mechanically more resistant than the commercial membranes. Number of fibroblasts seeded on sea-urchin membranes were comparable to the bovine collagen substrates. Cell morphology on all EDCMs was similar to that of structurally comparable (reassembled) bovine collagen substrates. Overall, echinoderms, and sea urchins particularly, are alternative collagen sources to produce efficient GTR membranes. Sea urchins display a further advantage in terms of eco-sustainability by recycling tissues from food wastes.
UR - http://hdl.handle.net/10754/604714
UR - http://linkinghub.elsevier.com/retrieve/pii/S0141113616300320
UR - http://www.scopus.com/inward/record.url?scp=84962761424&partnerID=8YFLogxK
U2 - 10.1016/j.marenvres.2016.03.007
DO - 10.1016/j.marenvres.2016.03.007
M3 - Article
C2 - 27063846
SN - 0141-1136
VL - 128
SP - 46
EP - 57
JO - Marine Environmental Research
JF - Marine Environmental Research
ER -