TY - JOUR
T1 - Marine Microorganisms as Source of Stereoselective Esterases and Ketoreductases: Kinetic Resolution of a Prostaglandin Intermediate
AU - De Vitis, Valerio
AU - Guidi, Benedetta
AU - Contente, Martina Letizia
AU - Granato, Tiziana Mariarita
AU - Conti, Paola
AU - Molinari, Francesco
AU - Crotti, Elena
AU - Mapelli, Francesca
AU - Borin, Sara S.
AU - Daffonchio, Daniele
AU - Romano, Diego
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: F. Mapelli was supported by the Universita degli Studi di Milano, DeFENS, European Social Fund (FSE) and Regione Lombardia (Contract "Dote Ricerca"). This work was supported by the European Community FP7-KBBE.2012.3.2-02 project MACUMBA, grant agreement N. 311975.
PY - 2014/9/30
Y1 - 2014/9/30
N2 - A screening among bacterial strains isolated from water-brine interface of the deep hypersaline anoxic basins (DHABs) of the Eastern Mediterranean was carried out for the biocatalytical resolution of racemic propyl ester of anti-2-oxotricyclo[2.2.1.0]heptan-7-carboxylic acid (R,S)-1, a key intermediate for the synthesis of d-cloprostenol. Bacillus horneckiae 15A gave highly stereoselective reduction of (R,S)-1, whereas Halomonas aquamarina 9B enantioselectively hydrolysed (R,S)-1; in both cases, enantiomerically pure unreacted (R)-1 could be easily recovered and purified at molar conversion below 57–58 %, showing the potential of DHAB extremophile microbiome and marine-derived enzymes in stereoselective biocatalysis.
AB - A screening among bacterial strains isolated from water-brine interface of the deep hypersaline anoxic basins (DHABs) of the Eastern Mediterranean was carried out for the biocatalytical resolution of racemic propyl ester of anti-2-oxotricyclo[2.2.1.0]heptan-7-carboxylic acid (R,S)-1, a key intermediate for the synthesis of d-cloprostenol. Bacillus horneckiae 15A gave highly stereoselective reduction of (R,S)-1, whereas Halomonas aquamarina 9B enantioselectively hydrolysed (R,S)-1; in both cases, enantiomerically pure unreacted (R)-1 could be easily recovered and purified at molar conversion below 57–58 %, showing the potential of DHAB extremophile microbiome and marine-derived enzymes in stereoselective biocatalysis.
UR - http://hdl.handle.net/10754/575616
UR - http://link.springer.com/10.1007/s10126-014-9602-z
UR - http://www.scopus.com/inward/record.url?scp=84925507234&partnerID=8YFLogxK
U2 - 10.1007/s10126-014-9602-z
DO - 10.1007/s10126-014-9602-z
M3 - Article
SN - 1436-2228
VL - 17
SP - 144
EP - 152
JO - Marine Biotechnology
JF - Marine Biotechnology
IS - 2
ER -
