TY - JOUR
T1 - Metabolomics of plant root exudates: From sample preparation to data analysis.
AU - Salem, Mohamed A
AU - Wang, Jian You
AU - Al-Babili, Salim
N1 - KAUST Repository Item: Exported on 2023-01-02
Acknowledgements: This work was supported by baseline funding given from King Abdullah University of Science and Technology (KAUST) and by the Bill and Melinda Gates Foundation grant OPP1194472 given to SA-B. We thank Dr. Justine Braguy for the artistic illustration of the presented figure.
PY - 2022/12/6
Y1 - 2022/12/6
N2 - Plants release a set of chemical compounds, called exudates, into the rhizosphere, under normal conditions and in response to environmental stimuli and surrounding soil organisms. Plant root exudates play indispensable roles in inhibiting the growth of harmful microorganisms, while also promoting the growth of beneficial microbes and attracting symbiotic partners. Root exudates contain a complex array of primary and specialized metabolites. Some of these chemicals are only found in certain plant species for shaping the microbial community in the rhizosphere. Comprehensive understanding of plant root exudates has numerous applications from basic sciences to enhancing crop yield, production of stress-tolerant crops, and phytoremediation. This review summarizes the metabolomics workflow for determining the composition of root exudates, from sample preparation to data acquisition and analysis. We also discuss recent advances in the existing analytical methods and future perspectives of metabolite analysis.
AB - Plants release a set of chemical compounds, called exudates, into the rhizosphere, under normal conditions and in response to environmental stimuli and surrounding soil organisms. Plant root exudates play indispensable roles in inhibiting the growth of harmful microorganisms, while also promoting the growth of beneficial microbes and attracting symbiotic partners. Root exudates contain a complex array of primary and specialized metabolites. Some of these chemicals are only found in certain plant species for shaping the microbial community in the rhizosphere. Comprehensive understanding of plant root exudates has numerous applications from basic sciences to enhancing crop yield, production of stress-tolerant crops, and phytoremediation. This review summarizes the metabolomics workflow for determining the composition of root exudates, from sample preparation to data acquisition and analysis. We also discuss recent advances in the existing analytical methods and future perspectives of metabolite analysis.
UR - http://hdl.handle.net/10754/686694
UR - https://www.frontiersin.org/articles/10.3389/fpls.2022.1062982/full
UR - http://www.scopus.com/inward/record.url?scp=85144334019&partnerID=8YFLogxK
U2 - 10.3389/fpls.2022.1062982
DO - 10.3389/fpls.2022.1062982
M3 - Article
C2 - 36561464
SN - 1664-462X
VL - 13
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
ER -