TY - JOUR
T1 - Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life
AU - Rimet, Frédéric
AU - Aylagas, Eva
AU - Borja, Ángel
AU - Bouchez, Agnès
AU - Canino, Alexis
AU - Chauvin, Christian
AU - Chonova, Teofana
AU - Ciampor Jr, Fedor
AU - Costa, Filipe O.
AU - Ferrari, Benoit J. D.
AU - Gastineau, Romain
AU - Goulon, Chloé
AU - Gugger, Muriel
AU - Holzmann, Maria
AU - Jahn, Regine
AU - Kahlert, Maria
AU - Kusber, Wolf-Henning
AU - Laplace-Treyture, Christophe
AU - Leese, Florian
AU - Leliaert, Frederik
AU - Mann, David G.
AU - Marchand, Frédéric
AU - Méléder, Vona
AU - Pawlowski, Jan
AU - Rasconi, Serena
AU - Rivera, Sinziana
AU - Rougerie, Rodolphe
AU - Schweizer, Magali
AU - Trobajo, Rosa
AU - Vasselon, Valentin
AU - Vivien, Régis
AU - Weigand, Alexander
AU - Witkowski, Andrzej
AU - Zimmermann, Jonas
AU - Ekrem, Torbjørn
N1 - KAUST Repository Item: Exported on 2021-03-03
Acknowledgements: Patricia Mergen (Botanical Garden of Meise, Belgium) is thanked for introducing experts who co-authored this paper. We thank Charlotte Duval and Claude Yéprémian of the MNHN (Muséum National d’Histoire Naturelle, Paris, France) for their comments. We acknowledge Axel Hausmann, Matthias F. Geiger and two additional anonymous reviewers for their constructive comments.
This paper was written under COST Action DNAqua-Net. The European COST Action DNAqua-Net (CA 15219 https://dnaqua.net/) is a collaborative network which gathers several hundreds of scientists and water managers, with the objective of developing new genetic tools for bioassessment and monitoring of aquatic ecosystems (Leese et al. 2016). Reference libraries, together with their feasibility and quality, are one of the most important subjects of DNAqua-Net and several meetings highlighted the need to improve the reference data and maintain their quality and reliability. This has been formalised in the discussion of Weigand et al. (2019).
PY - 2021/2/26
Y1 - 2021/2/26
N2 - DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.
AB - DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.
UR - http://hdl.handle.net/10754/667795
UR - https://mbmg.pensoft.net/article/58056/
U2 - 10.3897/mbmg.5.58056
DO - 10.3897/mbmg.5.58056
M3 - Article
SN - 2534-9708
VL - 5
JO - Metabarcoding and Metagenomics
JF - Metabarcoding and Metagenomics
ER -