Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon-contaminated soils in King George Island, Maritime Antarctica

Diogo Jurelevicius, Raphael da Silva Pereira, Fabio Faria Mota, Juliano C. Cury, Ivan Cardoso Oliveira, Alexandre S. Rosado, Olivia U. Mason, Janet K. Jansson, Lucy Seldin

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Soil samples from a transect from low to highly hydrocarbon-contaminated soils were collected around the Brazilian Antarctic Station Comandante Ferraz (EACF), located at King George Island, Antarctica. Quantitative PCR (qPCR) analysis of bacterial 16S rRNA genes, 16S rRNA gene (iTag), and shotgun metagenomic sequencing were used to characterize microbial community structure and the potential for petroleum degradation by indigenous microbes. Hydrocarbon contamination did not affect bacterial abundance in EACF soils (bacterial 16S rRNA gene qPCR). However, analysis of 16S rRNA gene sequences revealed a successive change in the microbial community along the pollution gradient. Microbial richness and diversity decreased with the increase of hydrocarbon concentration in EACF soils. The abundance of Cytophaga, Methyloversatilis, Polaromonas, and Williamsia was positively correlated (p-value = 
Original languageEnglish (US)
JournalGeobiology
DOIs
StatePublished - Sep 20 2021

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • General Environmental Science
  • General Earth and Planetary Sciences

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