TY - JOUR
T1 - Metagenomic analysis of the shrew enteric virome reveals novel viruses related to human stool-associated viruses
AU - Sasaki, Michihito
AU - Orba, Yasuko
AU - Ueno, Keisuke
AU - Ishii, Akihiro
AU - Moonga, Ladslav
AU - Hangombe, Bernard M.
AU - Mweene, Aaron S.
AU - Ito, Kimihito
AU - Sawa, Hirofumi
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Shrews are small insectivorous mammals that are distributed worldwide. Similar to rodents, shrews live on the ground and are commonly found near human residences. In this study, we investigated the enteric virome of wild shrews in the genus Crocidura using a sequence-independent viral metagenomics approach. A large portion of the shrew enteric virome was composed of insect viruses, whilst novel viruses including cyclovirus, picornavirus and picorna-like virus were also identified. Several cycloviruses, including variants of human cycloviruses detected in cerebrospinal fluid and stools, were detected in wild shrews at a high prevalence rate. The identified picornavirus was distantly related to human parechovirus, inferring the presence of a new genus in this family. The identified picorna-like viruses were characterized as different species of calhevirus 1, which was discovered previously in human stools. Complete or nearly complete genome sequences of these novel viruses were determined in this study and then were subjected to further genetic characterization. Our study provides an initial view of the diversity and distinctiveness of the shrew enteric virome and highlights unique novel viruses related to human stool-associated viruses.
AB - Shrews are small insectivorous mammals that are distributed worldwide. Similar to rodents, shrews live on the ground and are commonly found near human residences. In this study, we investigated the enteric virome of wild shrews in the genus Crocidura using a sequence-independent viral metagenomics approach. A large portion of the shrew enteric virome was composed of insect viruses, whilst novel viruses including cyclovirus, picornavirus and picorna-like virus were also identified. Several cycloviruses, including variants of human cycloviruses detected in cerebrospinal fluid and stools, were detected in wild shrews at a high prevalence rate. The identified picornavirus was distantly related to human parechovirus, inferring the presence of a new genus in this family. The identified picorna-like viruses were characterized as different species of calhevirus 1, which was discovered previously in human stools. Complete or nearly complete genome sequences of these novel viruses were determined in this study and then were subjected to further genetic characterization. Our study provides an initial view of the diversity and distinctiveness of the shrew enteric virome and highlights unique novel viruses related to human stool-associated viruses.
UR - http://www.scopus.com/inward/record.url?scp=84921766948&partnerID=8YFLogxK
U2 - 10.1099/vir.0.071209-0
DO - 10.1099/vir.0.071209-0
M3 - Article
C2 - 25381053
AN - SCOPUS:84921766948
SN - 0022-1317
VL - 96
SP - 440
EP - 452
JO - Journal of General Virology
JF - Journal of General Virology
IS - 2
ER -