Metal-conjugated affinity labels: A new concept to create enantioselective artificial metalloenzymes

Thomas Reiner; Dominik Jantke; Alexander N. Marziale; Andreas Raba; Jörg Eppinger

doi:10.1002/open.201200044

Metal-conjugated affinity labels: A new concept to create enantioselective artificial metalloenzymes

Thomas Reiner, Dominik Jantke, Alexander N. Marziale, Andreas Raba, Jörg Eppinger

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

How to train a protein: Metal-conjugated affinity labels were used to selectively position catalytically active metal centers in the binding pocket of proteases. The resulting artificial metalloenzymes achieve up to 82% e.r. in the hydrogenation of ketones. The modular setup enables a rapid generation of artificial metalloenzyme libraries, which can be adapted to a broad range of catalytic conditions. 2013 The Authors.

Original language	English (US)
Pages (from-to)	50-54
Number of pages	5
Journal	ChemistryOpen
Volume	2
Issue number	2
DOIs	https://doi.org/10.1002/open.201200044
State	Published - Feb 20 2013

ASJC Scopus subject areas

General Chemistry

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10.1002/open.201200044

https://repository.kaust.edu.sa/bitstream/10754/325430/1/Article-ChemistryO-Metal-conj-2013.pdf

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Metal-conjugated affinity labels: A new concept to create enantioselective artificial metalloenzymes

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