TY - JOUR
T1 - Methods for making induced pluripotent stem cells
T2 - Reprogramming à la carte
AU - González, Federico
AU - Boué, Stéphanie
AU - Belmonte, Juan Carlos Izpisúa
N1 - Funding Information:
We apologize to those authors whose publications cannot be mentioned here owing to space constraints. F.G. was partially supported by a fellowship from the Swiss National Science Foundation. Work in the laboratory of J.C.I.B. was supported by grants from the G. Harold and Leila Y. Mathers Charitable Foundation, the Cellex Foundation, MICINN and Sanofi-Aventis.
PY - 2011/4
Y1 - 2011/4
N2 - Pluripotent stem-cell lines can be obtained through the reprogramming of somatic cells from different tissues and species by ectopic expression of defined factors. In theory, these cells- known as induced pluripotent stem cells (iPSCs)- are suitable for various purposes, including disease modelling, autologous cell therapy, drug or toxicity screening and basic research. Recent methodological improvements are increasing the ease and efficiency of reprogramming, and reducing the genomic modifications required to complete the process. However, depending on the downstream applications, certain technologies have advantages over others. Here, we provide a comprehensive overview of the existing reprogramming approaches with the aim of providing readers with a better understanding of the reprogramming process and a basis for selecting the most suitable method for basic or clinical applications.
AB - Pluripotent stem-cell lines can be obtained through the reprogramming of somatic cells from different tissues and species by ectopic expression of defined factors. In theory, these cells- known as induced pluripotent stem cells (iPSCs)- are suitable for various purposes, including disease modelling, autologous cell therapy, drug or toxicity screening and basic research. Recent methodological improvements are increasing the ease and efficiency of reprogramming, and reducing the genomic modifications required to complete the process. However, depending on the downstream applications, certain technologies have advantages over others. Here, we provide a comprehensive overview of the existing reprogramming approaches with the aim of providing readers with a better understanding of the reprogramming process and a basis for selecting the most suitable method for basic or clinical applications.
UR - http://www.scopus.com/inward/record.url?scp=79952899995&partnerID=8YFLogxK
U2 - 10.1038/nrg2937
DO - 10.1038/nrg2937
M3 - Review article
C2 - 21339765
AN - SCOPUS:79952899995
SN - 1471-0056
VL - 12
SP - 231
EP - 242
JO - Nature Reviews Genetics
JF - Nature Reviews Genetics
IS - 4
ER -