Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers

Gerardo Perozziello, Patrizio Candeloro, Antonio De Grazia, Francesco Esposito, Marco Allione, Maria Laura Coluccio, Rossana Tallerico, Immanuel Valpapuram, Luca Tirinato, Gobind Das, Andrea Giugni, Bruno Torre, Pierangelo Veltri, Ulrich Kruhne, Giuseppe Della Valle, Enzo M. Di Fabrizio

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

In this work a Raman flow cytometer is presented. It consists of a microfluidic device that takes advantages of the basic principles of Raman spectroscopy and flow cytometry. The microfluidic device integrates calibrated microfluidic channels- where the cells can flow one-by-one -, allowing single cell Raman analysis. The microfluidic channel integrates plasmonic nanodimers in a fluidic trapping region. In this way it is possible to perform Enhanced Raman Spectroscopy on single cell. These allow a label-free analysis, providing information about the biochemical content of membrane and cytoplasm of the each cell. Experiments are performed on red blood cells (RBCs), peripheral blood lymphocytes (PBLs) and myelogenous leukemia tumor cells (K562). © 2015 Optical Society of America.
Original languageEnglish (US)
Pages (from-to)A180
JournalOptics Express
Volume24
Issue number2
DOIs
StatePublished - Dec 11 2015

Fingerprint

Dive into the research topics of 'Microfluidic device for continuous single cells analysis via Raman spectroscopy enhanced by integrated plasmonic nanodimers'. Together they form a unique fingerprint.

Cite this