TY - JOUR
T1 - Microfold-cell targeted surface engineered polymeric nanoparticles for oral immunization
AU - Malik, Basant
AU - Goyal, Amit K.
AU - Markandeywar, T. S.
AU - Rath, Goutam
AU - Zakir, Foziyah
AU - Vyas, Suresh P.
N1 - Generated from Scopus record by KAUST IRTS on 2023-10-12
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Present work was envisaged to develop novel M-cell targeted polymeric particles that are capable of protecting the antigen from harsh gastric conditions. Ulex europaeus agglutinin (UEA-1) lectin was anchored for selective delivery of antigen to gut-associated lymphoid tissue (GALT). In the present investigation, chitosan nanoparticles were prepared by ionic gelation followed by antigen (bovine serum albumin, BSA) adsorption. Developed nanoparticles were further coated by UEA-1 lectin conjugated alginate and characterized for size, shape, zeta-potential, entrapment efficiency, and in vitro release. The immunological response of the developed system were performed in Balb/c mice and compared with aluminium hydroxide gel-based conventional vaccine. Results indicated that immunization with UEA-1 lectin conjugated alginate-coated particles induces efficient systemic as well as mucosal immune responses against BSA compared to other formulations. Aluminium-based vaccine dominated throughout the study, while failed in case of mucosal antibody. Additionally, IgG1 and IgG2a isotypes were determined to confirm the TH1/TH2 mixed immune response. The developed formulation exhibited superior systemic response along with dominating mucosal immunity. These data demonstrate the potential of UEA-alginate-coated nanoparticles as effective delivery system via oral route. © 2012 Informa UK, Ltd.
AB - Present work was envisaged to develop novel M-cell targeted polymeric particles that are capable of protecting the antigen from harsh gastric conditions. Ulex europaeus agglutinin (UEA-1) lectin was anchored for selective delivery of antigen to gut-associated lymphoid tissue (GALT). In the present investigation, chitosan nanoparticles were prepared by ionic gelation followed by antigen (bovine serum albumin, BSA) adsorption. Developed nanoparticles were further coated by UEA-1 lectin conjugated alginate and characterized for size, shape, zeta-potential, entrapment efficiency, and in vitro release. The immunological response of the developed system were performed in Balb/c mice and compared with aluminium hydroxide gel-based conventional vaccine. Results indicated that immunization with UEA-1 lectin conjugated alginate-coated particles induces efficient systemic as well as mucosal immune responses against BSA compared to other formulations. Aluminium-based vaccine dominated throughout the study, while failed in case of mucosal antibody. Additionally, IgG1 and IgG2a isotypes were determined to confirm the TH1/TH2 mixed immune response. The developed formulation exhibited superior systemic response along with dominating mucosal immunity. These data demonstrate the potential of UEA-alginate-coated nanoparticles as effective delivery system via oral route. © 2012 Informa UK, Ltd.
UR - http://www.tandfonline.com/doi/full/10.3109/1061186X.2011.611516
UR - http://www.scopus.com/inward/record.url?scp=83155183262&partnerID=8YFLogxK
U2 - 10.3109/1061186X.2011.611516
DO - 10.3109/1061186X.2011.611516
M3 - Article
SN - 1061-186X
VL - 20
SP - 76
EP - 84
JO - Journal of Drug Targeting
JF - Journal of Drug Targeting
IS - 1
ER -
