Microholography applied to tissue characterization

Christian D. Depeursinge*, Emmanuel B. Haller, Olivier Coquoz, Ramiro Conde, Gert Bally

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


An in-situ holographic technique, involving the use of a flexible miniaturized endoscope (diameter less than 1 mm) coupled to a CCD camera, to record the hologram, has been developed for medical applications and more particularly in-vivo biopsy. The hologram is formed, by reflection, on the tip of a multimode, multicore fiber (MCF), sampled, and then treated electronically. The image is reconstructed numerically, providing more flexibility to the holographic process. Reconstructed images show the capability of the microendoscopic system to restore 3D informations of the observed scene. Our predictions and experimental results have shown that the hope to achieve tissue observations at the cellular level is realistic. Furthermore, the different sources of noise of the experimental device were analyzed and their influence on the quality of the reconstructed image quantified. Images of simple cell models such as epithelial cells easily taken in the oral cavity, have been taken and analyzed. The possibility of using the microholographic technique for in-vivo biopsy is discussed both from theoretical considerations and experimental observations.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsRinaldo Cubeddu, Renato Marchesini, Serge R. Mordon, Katarina Svanberg, Herbert H. Rinneberg, al et al
Number of pages8
StatePublished - 1995
Externally publishedYes
EventOptical Biopsy and Fluorescence Spectroscopy and Imaging - Lille, Fr
Duration: Sep 9 1994Sep 10 1994

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


OtherOptical Biopsy and Fluorescence Spectroscopy and Imaging
CityLille, Fr

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Applied Mathematics
  • Electrical and Electronic Engineering
  • Computer Science Applications


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