TY - JOUR
T1 - Microradiography as a tool to detect heavy metal uptake in plants for phytoremediation applications
AU - Reale, Lucia
AU - Lai, Antonia
AU - Bellucci, Ida
AU - Faenov, Anatoly
AU - Pikuz, Tatiana
AU - Flora, Francesco
AU - Spanò, Laura
AU - Poma, Anna
AU - Limongi, Tania
AU - Palladino, Libero
AU - Ritucci, Antonio
AU - Tomassetti, Giuseppe
AU - Petrocelli, Giovanni
AU - Martellucci, Sergio
PY - 2006/8
Y1 - 2006/8
N2 - In this paper, an application of contact microradiography with soft X-rays for detecting the uptake site of heavy metal in the whole plant leaves is investigated. The X-ray source is a laser-plasma one based on an Nd:glass laser. The soft X-ray radiation emitted from the plasma laser targets of magnesium, iron, and copper can be strongly absorbed in the leaves' regions rich in iron, magnesium, and copper. This absorbance could point to structures in the leaves where these heavy elements are found. In this work, leaves treated with copper sulfate diluted in water at 1, 2, and 5% were imaged by using a copper target, in order to evaluate differences with untreated control leaves. Our results showed that this methodology highlighted the presence of copper in the treated leaves. This new methodology should detect heavy element pollutants inside plants and it should also be a useful analytic tool in phytoremediation studies.
AB - In this paper, an application of contact microradiography with soft X-rays for detecting the uptake site of heavy metal in the whole plant leaves is investigated. The X-ray source is a laser-plasma one based on an Nd:glass laser. The soft X-ray radiation emitted from the plasma laser targets of magnesium, iron, and copper can be strongly absorbed in the leaves' regions rich in iron, magnesium, and copper. This absorbance could point to structures in the leaves where these heavy elements are found. In this work, leaves treated with copper sulfate diluted in water at 1, 2, and 5% were imaged by using a copper target, in order to evaluate differences with untreated control leaves. Our results showed that this methodology highlighted the presence of copper in the treated leaves. This new methodology should detect heavy element pollutants inside plants and it should also be a useful analytic tool in phytoremediation studies.
KW - Microradiography
KW - Phytoremediation
KW - Plant leaves
KW - X-rays
UR - http://www.scopus.com/inward/record.url?scp=33748333374&partnerID=8YFLogxK
U2 - 10.1002/jemt.20350
DO - 10.1002/jemt.20350
M3 - Article
C2 - 16788990
AN - SCOPUS:33748333374
SN - 1059-910X
VL - 69
SP - 666
EP - 674
JO - Microscopy Research and Technique
JF - Microscopy Research and Technique
IS - 8
ER -