TY - JOUR
T1 - mir-300 promotes self-renewal and inhibits the differentiation of glioma stem-like cells
AU - Zhang, Daming
AU - Yang, Guang
AU - Chen, Xin
AU - Li, Chunmei
AU - Wang, Lu
AU - Liu, Yaohua
AU - Han, Dayong
AU - Liu, Huailei
AU - Hou, Xu
AU - Zhang, Weiguang
AU - Li, Chenguang
AU - Han, Zhanqiang
AU - Gao, Xin
AU - Zhao, Shiguang
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the National Natural Science Foundation of China (grant numbers 81272788 and 81302178), the Natural Science Foundation of Heilongjiang (QC2013C096), and the Fund of the First Affiliated Hospital of Harbin Medical University (2013B01).
PY - 2014/1/28
Y1 - 2014/1/28
N2 - MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.
AB - MicroRNAs (miRNAs) are small noncoding RNAs that have been critically implicated in several human cancers. miRNAs are thought to participate in various biological processes, including proliferation, cell cycle, apoptosis, and even the regulation of the stemness properties of cancer stem cells. In this study, we explore the potential role of miR-300 in glioma stem-like cells (GSLCs). We isolated GSLCs from glioma biopsy specimens and identified the stemness properties of the cells through neurosphere formation assays, multilineage differentiation ability analysis, and immunofluorescence analysis of glioma stem cell markers. We found that miR-300 is commonly upregulated in glioma tissues, and the expression of miR-300 was higher in GSLCs. The results of functional experiments demonstrated that miR-300 can enhance the self-renewal of GSLCs and reduce differentiation toward both astrocyte and neural fates. In addition, LZTS2 is a direct target of miR-300. In conclusion, our results demonstrate the critical role of miR-300 in GSLCs and its functions in LZTS2 inhibition and describe a new approach for the molecular regulation of tumor stem cells. © 2014 Springer Science+Business Media.
UR - http://hdl.handle.net/10754/563358
UR - http://link.springer.com/10.1007/s12031-014-0230-x
UR - http://www.scopus.com/inward/record.url?scp=84905111933&partnerID=8YFLogxK
U2 - 10.1007/s12031-014-0230-x
DO - 10.1007/s12031-014-0230-x
M3 - Article
SN - 0895-8696
VL - 53
SP - 637
EP - 644
JO - Journal of Molecular Neuroscience
JF - Journal of Molecular Neuroscience
IS - 4
ER -
