Abstract
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. © 2013 John Wiley & Sons Ltd.
Original language | English (US) |
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Pages (from-to) | 74-80 |
Number of pages | 7 |
Journal | International Journal of Immunogenetics |
Volume | 41 |
Issue number | 1 |
DOIs | |
State | Published - Jun 25 2013 |
ASJC Scopus subject areas
- Immunology
- Genetics
- Molecular Biology
- Genetics(clinical)