Abstract
Molecular defects in Factor VIII (FVIII), such as haemophilia A-related mutations or denaturative conformational changes, may affect the stability of FVIII as well as its interactions with physiological activators, von Willebrand Factor, phospholipid, or conformationally sensitive antibodies. We summarize the contemporary assays which allow identification of impaired functional interactions of FVIII that cause a reduction or loss of its cofactor activity and/or increased immunogenicity. These assays can potentially be used for detection of molecular defects in FVIII and elucidation of the function impaired by these defects.
Original language | English (US) |
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Pages (from-to) | 89-96 |
Number of pages | 8 |
Journal | Vox Sanguinis |
Volume | 83 |
Issue number | 2 |
DOIs | |
State | Published - Aug 2002 |
Externally published | Yes |
Keywords
- Conformational changes
- Factor VIII
- Haemophilia A
- Mutations
- Phospholipid binding
- Surface plasmon resonance
ASJC Scopus subject areas
- Hematology