TY - JOUR
T1 - Molecular evidence for multiple infections as revealed by typing of asaia bacterial symbionts of four mosquito species
AU - Chouaia, Bessem
AU - Rossi, Paolo
AU - Montagna, Matteo
AU - Ricci, Irene
AU - Crotti, Elena
AU - Damiani, Claudia
AU - Epis, Sara
AU - Faye, Ingrid
AU - Sagnon, N'Fale
AU - Alma, Alberto
AU - Favia, Guido
AU - Daffonchio, Daniele
AU - Bandi, Claudio
PY - 2010/11
Y1 - 2010/11
N2 - The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -independent techniques, we investigated the microbiota associated with four mosquito species, Anopheles stephensi, Anopheles gambiae, Aedes aegypti, and Aedes albopictus, which are important vectors of human and/or animal pathogens. Denaturing gradient gel electrophoresis (DGGE) analysis based on the 16S rRNA gene revealed the presence of several bacterial taxa, among which Asaia sequences were among the dominant in most of the samples. A collection of 281 Asaia isolates in cell-free media was established from individuals belonging to the four species. The isolates were typed by internal transcribed spacer (ITS)-PCR, tRNA-PCR, BOX-PCR, and randomly amplified polymorphic DNA (RAPD)-PCR, revealing that different Asaia strains are present in different mosquito populations, and even in single individuals.
AB - The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -independent techniques, we investigated the microbiota associated with four mosquito species, Anopheles stephensi, Anopheles gambiae, Aedes aegypti, and Aedes albopictus, which are important vectors of human and/or animal pathogens. Denaturing gradient gel electrophoresis (DGGE) analysis based on the 16S rRNA gene revealed the presence of several bacterial taxa, among which Asaia sequences were among the dominant in most of the samples. A collection of 281 Asaia isolates in cell-free media was established from individuals belonging to the four species. The isolates were typed by internal transcribed spacer (ITS)-PCR, tRNA-PCR, BOX-PCR, and randomly amplified polymorphic DNA (RAPD)-PCR, revealing that different Asaia strains are present in different mosquito populations, and even in single individuals.
UR - http://www.scopus.com/inward/record.url?scp=78649690170&partnerID=8YFLogxK
U2 - 10.1128/AEM.01747-10
DO - 10.1128/AEM.01747-10
M3 - Article
C2 - 20851960
AN - SCOPUS:78649690170
SN - 0099-2240
VL - 76
SP - 7444
EP - 7450
JO - APPLIED AND ENVIRONMENTAL MICROBIOLOGY
JF - APPLIED AND ENVIRONMENTAL MICROBIOLOGY
IS - 22
ER -