TY - JOUR
T1 - Molecular Targets and Biological Functions of cAMP Signaling in Arabidopsis
AU - Xu, Ruqiang
AU - Guo, Yanhui
AU - Peng, Song
AU - Liu, Jinrui
AU - Li, Panyu
AU - Jia, Wenjing
AU - Zhao, Junheng
N1 - KAUST Repository Item: Exported on 2021-05-10
Acknowledgements: The authors wish to thank Chris Gehring from King Abdullah University of Science and Technology, Saudi Arabia, for providing pDEST17-AtKUP71−100 plasmid. The Gateway@-compatible vector pTA7001-DEST was a gift from Roger Innes (Addgene plasmid # 71745; http:
//n2t.net/addgene:71745 (accessed on 20 March 2021); RRID: Addgene_71745).
This publication acknowledges KAUST support, but has no KAUST affiliated authors.
PY - 2021/5/3
Y1 - 2021/5/3
N2 - Cyclic AMP (cAMP) is a pivotal signaling molecule existing in almost all living organisms. However, the mechanism of cAMP signaling in plants remains very poorly understood. Here, we employ the engineered activity of soluble adenylate cyclase to induce cellular cAMP elevation in Arabidopsis thaliana plants and identify 427 cAMP-responsive genes (CRGs) through RNA-seq analysis. Induction of cellular cAMP elevation inhibits seed germination, disturbs phytohormone contents, promotes leaf senescence, impairs ethylene response, and compromises salt stress tolerance and pathogen resistance. A set of 62 transcription factors are among the CRGs, supporting a prominent role of cAMP in transcriptional regulation. The CRGs are significantly overrepresented in the pathways of plant hormone signal transduction, MAPK signaling, and diterpenoid biosynthesis, but they are also implicated in lipid, sugar, K+, nitrate signaling, and beyond. Our results provide a basic framework of cAMP signaling for the community to explore. The regulatory roles of cAMP signaling in plant plasticity are discussed.
AB - Cyclic AMP (cAMP) is a pivotal signaling molecule existing in almost all living organisms. However, the mechanism of cAMP signaling in plants remains very poorly understood. Here, we employ the engineered activity of soluble adenylate cyclase to induce cellular cAMP elevation in Arabidopsis thaliana plants and identify 427 cAMP-responsive genes (CRGs) through RNA-seq analysis. Induction of cellular cAMP elevation inhibits seed germination, disturbs phytohormone contents, promotes leaf senescence, impairs ethylene response, and compromises salt stress tolerance and pathogen resistance. A set of 62 transcription factors are among the CRGs, supporting a prominent role of cAMP in transcriptional regulation. The CRGs are significantly overrepresented in the pathways of plant hormone signal transduction, MAPK signaling, and diterpenoid biosynthesis, but they are also implicated in lipid, sugar, K+, nitrate signaling, and beyond. Our results provide a basic framework of cAMP signaling for the community to explore. The regulatory roles of cAMP signaling in plant plasticity are discussed.
UR - http://hdl.handle.net/10754/669127
UR - https://www.mdpi.com/2218-273X/11/5/688
U2 - 10.3390/biom11050688
DO - 10.3390/biom11050688
M3 - Article
C2 - 34063698
SN - 2218-273X
VL - 11
SP - 688
JO - Biomolecules
JF - Biomolecules
IS - 5
ER -