Multimer resolution systems of ColE1 and ColK: localisation of the crossover site

David Summers; Sami Yaish; John Archer; David Sherratt

doi:10.1007/BF00425680

Multimer resolution systems of ColE1 and ColK: localisation of the crossover site

David Summers^*, Sami Yaish, John Archer, David Sherratt

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We have identified and characterised a stability function encoded by the high copy plasmid ColK. The function is analogous to ColE1 cer and maximises stability by maintaining plasmids in the monomeric state. In vivo recombination between cer and ckr (which share more than 90% homology at the DNA sequence level) produced a functional hybrid. Sequence analysis of hybrids indicates that recombination involving cer and ckr is site-specific and occurs within a 35 bp region of DNA which contains palindromic symmetry.

Original language	English (US)
Pages (from-to)	334-338
Number of pages	5
Journal	MGG Molecular & General Genetics
Volume	201
Issue number	2
DOIs	https://doi.org/10.1007/BF00425680
State	Published - Nov 1985
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1007/BF00425680

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title = "Multimer resolution systems of ColE1 and ColK: localisation of the crossover site",

abstract = "We have identified and characterised a stability function encoded by the high copy plasmid ColK. The function is analogous to ColE1 cer and maximises stability by maintaining plasmids in the monomeric state. In vivo recombination between cer and ckr (which share more than 90% homology at the DNA sequence level) produced a functional hybrid. Sequence analysis of hybrids indicates that recombination involving cer and ckr is site-specific and occurs within a 35 bp region of DNA which contains palindromic symmetry.",

author = "David Summers and Sami Yaish and John Archer and David Sherratt",

year = "1985",

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doi = "10.1007/BF00425680",

language = "English (US)",

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T2 - localisation of the crossover site

AU - Summers, David

AU - Yaish, Sami

AU - Archer, John

AU - Sherratt, David

PY - 1985/11

Y1 - 1985/11

N2 - We have identified and characterised a stability function encoded by the high copy plasmid ColK. The function is analogous to ColE1 cer and maximises stability by maintaining plasmids in the monomeric state. In vivo recombination between cer and ckr (which share more than 90% homology at the DNA sequence level) produced a functional hybrid. Sequence analysis of hybrids indicates that recombination involving cer and ckr is site-specific and occurs within a 35 bp region of DNA which contains palindromic symmetry.

AB - We have identified and characterised a stability function encoded by the high copy plasmid ColK. The function is analogous to ColE1 cer and maximises stability by maintaining plasmids in the monomeric state. In vivo recombination between cer and ckr (which share more than 90% homology at the DNA sequence level) produced a functional hybrid. Sequence analysis of hybrids indicates that recombination involving cer and ckr is site-specific and occurs within a 35 bp region of DNA which contains palindromic symmetry.

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U2 - 10.1007/BF00425680

DO - 10.1007/BF00425680

M3 - Article

C2 - 3003536

AN - SCOPUS:0022383367

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JO - MGG Molecular & General Genetics

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ER -

Multimer resolution systems of ColE1 and ColK: localisation of the crossover site

Abstract

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