Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

Patrizio Candeloro; Luca Tirinato; Natalia Malara; Annalisa Fregola; Eudald Casals; Victor Puntes; Gerardo Perozziello; Francesco Gentile; Maria Laura Coluccio; Gobind Das; Carlo Liberale; Francesco De Angelis; Enzo Di Fabrizio

doi:10.1039/c1an15313g

Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

Patrizio Candeloro^*, Luca Tirinato, Natalia Malara, Annalisa Fregola, Eudald Casals, Victor Puntes, Gerardo Perozziello, Francesco Gentile, Maria Laura Coluccio, Gobind Das, Carlo Liberale, Francesco De Angelis, Enzo Di Fabrizio

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe ₃O₄ nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.

Original language	English (US)
Pages (from-to)	4402-4408
Number of pages	7
Journal	Analyst
Volume	136
Issue number	21
DOIs	https://doi.org/10.1039/c1an15313g
State	Published - Nov 7 2011
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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title = "Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies",

abstract = "Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe 3O4 nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.",

author = "Patrizio Candeloro and Luca Tirinato and Natalia Malara and Annalisa Fregola and Eudald Casals and Victor Puntes and Gerardo Perozziello and Francesco Gentile and Coluccio, {Maria Laura} and Gobind Das and Carlo Liberale and {De Angelis}, Francesco and {Di Fabrizio}, Enzo",

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doi = "10.1039/c1an15313g",

language = "English (US)",

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TY - JOUR

T1 - Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

AU - Candeloro, Patrizio

AU - Tirinato, Luca

AU - Malara, Natalia

AU - Fregola, Annalisa

AU - Casals, Eudald

AU - Puntes, Victor

AU - Perozziello, Gerardo

AU - Gentile, Francesco

AU - Coluccio, Maria Laura

AU - Das, Gobind

AU - Liberale, Carlo

AU - De Angelis, Francesco

AU - Di Fabrizio, Enzo

PY - 2011/11/7

Y1 - 2011/11/7

N2 - Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe 3O4 nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.

AB - Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe 3O4 nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.

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Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies

Abstract

ASJC Scopus subject areas

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